Determination of the complete genomic DNA sequence of Thermoplasma volcanium GSS1
スポンサーリンク
概要
- 論文の詳細を見る
The complete genomic DNA sequence of the aero/anaero-facultative archaebacterium, Thermoplasma volcanium GSS1, has been determined. A number of DNA fragments were cloned by using the λ, cosmid, and BAC systems, and sequenced. The remaining 30 gaps were bridged by DNA fragments constructed using the polymerase chain reaction. The repetition in sequencing the same base positions was 13.1±7.5 fold. The alignment of the DNA fragments and the completeness of the genomic sequence were confirmed by the consistency of the genomic sequence with the lengths and partial sequences of a second set of DNA fragments that altogether covered 88% of the genome. The number of bases found in the genomic sequence is 1, 584, 799, with a G/C content of 39.9%. The combination of the four types of bases in the new genomic sequence is compared with those in known genomic sequences of similar sizes.
- 日本学士院の論文
著者
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Aramaki Hironori
Department Of Microbiology Daiichi College Of Pharmaceutical Sciences:faculty Of Pharmaceutical Scie
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Kawamoto Takeshi
Department Of Anesthesiology Kansai Denryoku Hospital
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NUNOSHIBA TATSUO
Department of Biomolecular Sciences, Graduate School of Life Sciences, Tohoku University
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Suzuki Masashi
AIST-NIBHT Structural Biology Centre
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YAMAZAKI Masaaki
Bioscience Lab. Fujiya.
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Makino Kozo
Department Of Applied Chemistry National Defense Academy
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Aramaki H
Department Of Molecular Biology Daiichi College Of Pharmaceutical Sciences
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Yamamoto Yoshihiro
Department Of Biological Science Graduate School Of Science Hiroshima University
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KAWASHIMA Tsuyoshi
AIST-NIBHT CREST Centre of Structural Biology
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AMANO Naoki
AIST-NIBHT CREST Centre of Structural Biology
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Kawashima T
National Institute Of Advanced Industrial Science And Technology:japan Science And Technology Agency
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WATANABE Kohji
Bioscience Research Laboratory
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KANEHORI Keiichi
DNA Analysis Department, Techno Research Laboratory, Hitachi Science Systems, Ltd.
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OHYA Yoshie
AIST-NIBHT CREST Centre of Structural Biology
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KAWAMOTO Takeshi
Department of Biochemistry, Hiroshima University, School of Dentistry
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MAKINO Kozo
Department of Molecular Microbiology, The Research Institute of Microbial Diseases, Osaka University
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YAMAZAKI Masaaki
Bioscience Research Laboratory
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SUZUKI Masashi
AIST-NIBHT CREST Centre of Structural Biology
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