Selenite Assimilation into Formate Dehydrogenase H Depends on Thioredoxin Reductase in Escherichia coli
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概要
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<p>Escherichia coli growing under anaerobic conditions produce H-2 and CO2 by the enzymatic cleavage of formate that is produced from pyruvate at the end of glycolysis. Selenium is an integral part of formate dehydrogenase H (FDHH), which catalyses the first step in the formate hydrogen lyase (FHL) system. The genes of FHL system are transcribed only under anaerobic conditions, in the presence of a sigma(54)-dependent transcriptional activator Fh1A that binds formate as an effector molecule. Although the formate addition to the nutrient media has been an established procedure for inducing high FDHH activity, we have identified a low-salt nutrient medium containing <0.1% NaCl enabled constitutive, high expression of FDHH even without formate and D-glucose added to the medium. The novel conditions allowed us to study the effects of disrupting genes like trxB (thioredoxin reductase) or gor (glutathione reductase) on the production of FDHH activity and also reductive assimilation of selenite (SeO32-) into the selenoprotein. Despite the widely accepted hypothesis that selenite is reduced by glutathione reductase-dependent system, it was demonstrated that trxB gene was essential for FDHH production and for labelling the FDHH polypeptide with Se-75-selenite. Our present study reports for the first time the physiological involvement of thioredoxin reductase in the reductive assimilation of selenite in E. coli.</p>
- 2008-04-01
著者
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Esaki N
Institute For Chemical Research Kyoto University
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Esaki Nobuyoshi
Kyoto Univ.
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Esaki Nobuyoshi
Nstitute For Chemical Research Kyoto University
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Esaki Nobuyoshi
京都大学化学研究所 分子微生物科学
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Ezaki Nobuyoshi
Laboratory Of Microbial Biochemistry Institute For Chemical Research Kyoto University
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Tamura Takashi
Department of Bioresources Chemistry, Graduate School of Natural Science and Technology, Okayama Uni
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Inagaki Kenji
Department of Bioresources Chemistry, Graduate School of Natural Science and Technology, Okayama Uni
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Yamamoto Yoshihiro
Department of Genetics, Hyogo College of Medicine
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Esaki Nobuyoshi
Inst. For Chemical Res. Kyoto Univ.
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Mizuno Haruo
Departments Of Pediatrics And Neonatology Nagoya City University Graduate School Of Medical Sciences
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Mizuno Haruo
Department Of Biotechnology Graduate School Of Engineering Osaka University
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Furutani Y
Okayama Univ. Okayama Jpn
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Inagaki Kenji
Dep. Of Bioresources Chemistry Graduate School Of Natural Sci. And Technol. Okayama Univ. 1-1-1 Tsus
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Inagaki Kenji
Department Of Bioresources Chemistry Graduate School Of Natural Science And Technology Okayama Unive
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Inagaki Kenji
Department Of Biofunctional Chemistry Graduate School Of Natural Science And Technology Okayama Univ
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Mizutani H
Dep. Of Biotechnology Coll. Of Life Sciences Ritsumeikan Univ.
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Tamura T
Department Of Biofunctional Chemistry Graduate School Of Natural Science And Technology Okayama Univ
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KUROKAWA Suguru
Laboratory of Molecular Microbial Science, Institute for Chemical Research, Kyoto University
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MIHARA Hisaaki
Laboratory of Molecular Microbial Science, Institute for Chemical Research, Kyoto University
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ABE Katsumasa
Institute for Chemical Research, Kyoto University
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Kurokawa Suguru
Institute For Chemical Research Kyoto University
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Abe Katsumasa
Institute For Chemical Research Kyoto University
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Esaki Nobuyoshi
Department Of Genetics Hyogo College Of Medicine
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Esaki N
Kyoto Univ.
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Kato Shinichiro
Institute For Chemical Research Kyoto University
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Esaki Nobuyoshi
Kyoto Univ. Kyoto Jpn
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Esaki Nobuyoshi
Institute For Chemical Resarch Kyoto University
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Mizutani Hisashi
Department Of Chemistry Faculty Of Science Osaka City University
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Matsubara Hiroshi
Institute For Chemical Research Kyoto University
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Kato S
Institute For Chemical Research Kyoto University
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Takahata Muneaki
Department Of Biofunctional Chemistry Graduate School Of Natural Science And Technology Okayama Univ
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Mizuguchi H
Department Of Biochemistry Osaka Medical College
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Tamura Takashi
Department Of Applied Chemistry Faculty Of Science Science University Of Tokyo
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ABE Katsumasa
Laboratory of Molecular Microbial Science, Institute for Chemical Research, Kyoto University
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NAKANO Ryuhei
Department of Biofunctional Chemistry, Graduate School of Natural Science and Technology, Okayama Un
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Murai Hiroki
名古屋市立大学 医学研究科新生児・小児医学分野
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Nakano Ryuhei
Department Of Biofunctional Chemistry Graduate School Of Natural Science And Technology Okayama Univ
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Yamamoto Yoshihiro
Department Of Biological Science Graduate School Of Science Hiroshima University
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Murai Hiroki
Department Of Pediatrics And Neonatology Nagoya City University Graduate School Of Medical Sciences
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Inagaki Kenji
Department Of Bioresources Chemistry Graduate School Of Natural Science And Technology Okayama Unive
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Inagaki K
Department Of Bioresources Chemistry Graduate School Of Natural Science And Technology Okayama Unive
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INAGAKI KENJI
Research Institute for Wakan-Yaku, Toyama Medical and Pharmaceutical University
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