The C_3-N Bond Cleavage of 2-Amino-3-(N-substituted-amino)-propionic Acids Catalyzed by L-Methionine γ-Lyase(Biological Chemistry)

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概要

• 論文の詳細を見る

• L-Methionine γ-lyase (EC 4.4.1.11) catalyzes α,β-elimination of L-2-amino-3-(N-methyl-amino)propionic acid and L-2-amino-3-(N-hydroxyethylamino)propionic acid to yield pyruvate, ammonia, and the corresponding amines. These amino acids also undergo the enzymatic β-re-placement reaction with thiols to produce the corresponding S-substituted cysteines. Thus, L-methionine-γ-lyase cleaves a C-N bond in addition to C-S, S-Se, and C-O bonds at the βposition of amino acids by elimination and replacement reactions. A linear relationship between the reactivity, (log(V_<max>/Km) and the pKa value of the conjugated acid of the leaving group has been found for Se-methyl-L-selenocysteine, S-methyl-L-cysteine, and O-methyl-L-serine. However, L-2-amino-3-(N-methylamino)propionic acid has shown lower reactivity than that expected from the pKa value of methylammonium ions.

• 社団法人日本農芸化学会の論文
• 1988-11-23

著者

• Esaki Nobuyoshi

  Kyoto Univ.

• Esaki Nobuyoshi

  Nstitute For Chemical Research Kyoto University

• TANAKA Hidehiko

  Institute for Chemical Research, Kyoto University

• Soda Kenji

  Institute For Chemical Resarch Kyoto University

• Takada Harumi

  Institute for Chemical Research, Kyoto University

• Esaki Nobuyoshi

  Institute For Chemical Resarch Kyoto University

• Soda K

  Department Of Biotechnology Kansai University

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