DnaK from Vibrio proteolyticus : Complementation of a dnaK-Null Mutant of Escherichia coli and the Role of Its ATPase Domain (Enzymology, Protein Engineering, and Enzyme Technology)

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概要

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• We cloned the 4.8-kbp DNA fragment containing the dnaK gene from the chromosomal DNA of Vibrio proteolyticus. It contained four genes arranged unidirectionally in the order of grpE, gltP, dnaK and dnaJ. The DnaK gene of V. proteolyticus (VprDnaK) allowed a dnaK-null mutant of Escherichia coli (ΔdnaK52) to propagateλphages but not to grow at 43℃. However, a chimeric DnaK gene comprising the regions corresponding to the N-terminal ATPase domain of E. coli DnaK (EcoDnaK) and the C-terminal region of VprDnaK including the substrate-binding domain, enabled the mutant to grow at 43℃. The temperature dependence for the ATPase activity of the chimeric DnaK was similar to that of EcoDnaK. Fluorometric analyses showed that the chimeric DnaK is much more thermostable than EcoDnaK and VprDnaK. These findings indicate that the thermal stability of the ATPase domain of DnaK is responsible for its chaperone action at high temperatures such as 43℃.

• 社団法人日本生物工学会の論文
• 2005-02-25

著者

• Esaki N

  Institute For Chemical Research Kyoto University

• Esaki Nobuyoshi

  Kyoto Univ.

• Esaki Nobuyoshi

  Nstitute For Chemical Research Kyoto University

• Esaki Nobuyoshi

  京都大学化学研究所 分子微生物科学

• Ezaki Nobuyoshi

  Laboratory Of Microbial Biochemistry Institute For Chemical Research Kyoto University

• Esaki Nobuyoshi

  Institute for Chemical Research, Kyoto University

• YOSHIMURA Tohru

  Institute for Chemical Research, Kyoto University

• Esaki Nobuyoshi

  Inst. For Chemical Res. Kyoto Univ.

• GALKIN Andrey

  Institute for Chemical Resarch, Kyoto University

• YOSHIMUNE KAZUAKI

  Institute for Chemical Research, Kyoto University

• KULAKOVA LJUDMILA

  Institute for Chemical Research, Kyoto University

• Esaki N

  Kyoto Univ.

• Yoshimura Tohru

  Institute For Chemical Research Kyoto University:(present Address)department Of Applied Molecular Bi

• Esaki Nobuyoshi

  Kyoto Univ. Kyoto Jpn

• Esaki Nobuyoshi

  Institute For Chemical Resarch Kyoto University

• Kulakova Ljudmila

  Institute For Chemical Research Kyoto University

• Galkin A

  Institute For Chemical Research Kyoto University

• Yoshimune Kazuaki

  Institute For Chemical Research Kyoto University:(present Address)department Of Applied Chemistry Fa

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