Primary Structure and Catalytic Properties of a Cold-active Esterase from a Psychrotroph, Acinetobacter sp. Strain No.6. Isolated from Siberian Soil(Biochemistry & Molecular Biology)
スポンサーリンク
概要
- 論文の詳細を見る
We cloned a gene coding for a cold-active esterase from a genomic library of Acinetobacter sp. strain No.6, a psychrotroph isolated from Siberian soil. The gene, aest, encoded a protein of 301 amino acid residues, the deduced sequence of which had less than 17% identity to sequences of known esterases and lipases. However, the esterase seemed to belong to the α/β hydrolase superfamily, because it contained a sequence, Gly-Xaa-Ser-Xaa-Gly (with Xaa an arbitrary amino acid residue), found in most serine hydrolases of this superfamily. Sequence comparison earlier suggested a weak phylogenetic relationship of gene product AEST to the EST group of the esterase-lipase family, which has been found only in eukaryotes. The aest gene was expressed in Escherichia coli BL21(DE3) cells under the control of the T7 promoter, and the expression product was purified to homogeneity and characterized. It catalyzed the hydrolysis of esters with short-chain acyl groups and had lower activation energy and lower thermostability than do mesophilic enzymes, as expected from the cold-adapted nature of this enzyme.
- 社団法人日本農芸化学会の論文
- 2002-08-23
著者
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Nishino Tokuzo
Department of Biomolecular Engineering, Graduate School of Engineering, Tohoku University
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Nakayama Toru
Department of Biomolecular Engineering, Graduate School of Engineering, Tohoku University
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Nakayama T
Department Of Biomolecular Engineering Graduate School Of Engineering Tohoku University
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Nishino T
Department Of Biochemistry And Engineering Faculty Of Engineering Tohoku University
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Nishino T
Department Of Biomolecular Engineering Graduate School Of Engineering Tohoku University
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Kurihara Tatsuo
京都大学化学研究所 分子微生物科学
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Kurihara T
Laboratory Of Applied Biological Chemistry Department Of Synthetic Chemistry And Biological Chemistr
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Kurihara Tatsuo
Laboratory Of Microbial Biochemistry Institute For Chemical Research Kyoto University
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Esaki N
Institute For Chemical Research Kyoto University
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Esaki Nobuyoshi
Kyoto Univ.
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Esaki Nobuyoshi
Nstitute For Chemical Research Kyoto University
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Esaki Nobuyoshi
京都大学化学研究所 分子微生物科学
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Ezaki Nobuyoshi
Laboratory Of Microbial Biochemistry Institute For Chemical Research Kyoto University
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Esaki Nobuyoshi
Laboratory Of Microbial Biochemistry Institute For Chemical Research Kyoto University
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Kurihara Tatsuo
Institute for Chemical Research, Kyoto University
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Kurihara T
Institute For Chemical Research Kyoto University
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SUZUKI Takeshi
Laboratory of Soil Science, Faculty of Agriculture, Kobe University
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Nishino T
Tohoku Univ. Miyagi Jpn
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Nishino Tokuzo
Dep. Of Biochemistry And Engineering Graduate School Of Engineering Tohoku Univ. Aoba-yama Sendai Mi
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Esaki Nobuyoshi
Inst. For Chemical Res. Kyoto Univ.
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Nakayama Toru
Department Of Biomolecular Engineering Graduate School Of Engineering Tohoku University
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Suzuki T
Research Center Sony Corporation
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Esaki N
Kyoto Univ.
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Kurihara T
Kyoto Univ. Kyoto
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Esaki Nobuyoshi
Kyoto Univ. Kyoto Jpn
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Esaki Nobuyoshi
Institute For Chemical Resarch Kyoto University
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Suzuki Tetsutaro
Departments Of Surgery Kitasato University School Of Medicine
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Suzuki T
Laboratory Of Microbial Biochemistry Institute For Chemical Research Kyoto University
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Nishino Tokuzo
Department Of Biochemistry And Engineering Faculty Of Engineering Tohoku University
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Suzuki Takeshi
Laboratory Of Soil Science Faculty Of Agriculture Kobe University
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Suzuki T
Departments Of Surgery Kitasato University School Of Medicine
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Suzuki Toshihide
Compound Semiconductor Devices Lab Fujitsu Laboratories Ltd.
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Suzuki Takeshi
Laboratory Of Microbial Biochemistry Institute For Chemical Research Kyoto University
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NAKAYAMA Toru
Department of Applied Microbial Technology, The Kumamoto Institute of Technology:(Present office)Institute for Fundamental Research, Suntory Ltd.
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