Identification of a cDNA Encoding Malonyl-Coenzyme A: Anthocyanidin 3-O-Glucoside 6"-O- Malonyltransferase from Cineraria (Senecio cruentus) Flowers

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概要

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• “Cinerarin” is a polyacylated anthocyanin that is responsible for the blue coloration of cineraria (Senecio cruentus) flowers. We isolated a full-length cDNA (Sc3MaT) encoding a putative anthocyanin acyltransferase from S. cruentus. The Sc3MaT cDNA was expressed in Escherichia coli cells and the expression product was purified to homogeneity and functionally characterized. The Sc3MaT could catalyze the regiospecific malonyl transfer from malonyl-CoA (Km, 61μM) to pelargonidin 3-O-glucoside (Km, 11μM) to produce pelargonidin 3-O-6”-O-malonylglucoside with a kcat value of 8.8 s-1. The specificities for acyl donors and acceptors were highly restricted to malonyl-CoA and anthocyanidin 3-O-glucoside, respectively. Therefore, it may be concluded that Sc3MaT is a malonyl-CoA:anthocyanidin 3-O-glucoside-6”-O-malonyltransferase. The other enzymatic properties of Sc3MaT were comparable with those of known anthocyanin acyltransferases. Because a reaction product of Sc3MaT, delphinidin 3-O-6”-O-malonyglucoside, constitutes a part of cinerarin, Sc3MaT is probably involved in the cinerarin biosynthesis in this plant.

• 日本植物細胞分子生物学会の論文
• 2003-09-01

著者

• Nishino Tokuzo

  Department of Biomolecular Engineering, Graduate School of Engineering, Tohoku University

• Nakayama Toru

  Department of Biomolecular Engineering, Graduate School of Engineering, Tohoku University

• Nishino Tokuzo

  Department Of Biomolecular Engineering Graduate School Of Engineering Tohoku University

• Nishino Tokuzo

  Tohoku Univ. Sendai Jpn

• Nishino Tokuzo

  Dep. Of Biochemistry And Engineering Graduate School Of Engineering Tohoku Univ. Aoba-yama Sendai Mi

• Nakayama Toru

  Department Of Biomolecular Engineering Graduate School Of Engineering Tohoku University

• SUZUKI Hirokazu

  Department of Medicinal Chemistry, Faculty of Pharmaceutical Sciences, Hokuriku University

• SAWADA Shin-ya

  Department of Biomolecular Engineering, Graduate School of Engineering, Tohoku University

• YONEKURA(SAKAKIBARA) Keiko

  Plant Science Center, RIKEN (The Institute of Physical and Chemical Research

• YAMAGUCHI Masa-atsu

  Department of Food Science and Technology, Minami-Kyushu University

• Suzuki Hirokazu

  Department Of Biomolecular Engineering Graduate School Of Engineering Tohoku University

• Yonekura(sakakibara) Keiko

  Plant Science Center Riken (the Institute Of Physical And Chemical Research

• Sawada Shin-ya

  Department Of Biomolecular Engineering Graduate School Of Engineering Tohoku University

• Nishino Tokuzo

  Department Of Biochemistry And Engineering Faculty Of Engineering Tohoku University

• Yamaguchi Masa-atsu

  Department Of Food Science And Technology College Of Horticulture Minami-kyushu University

• Yamaguchi Masa-atsu

  Department Of Food Science And Technology Minami-kyushu University

• Suzuki Hirokazu

  Department Of Applied Chemistry College Of Engineering Chubu University

• NAKAYAMA Toru

  Department of Applied Microbial Technology, The Kumamoto Institute of Technology:(Present office)Institute for Fundamental Research, Suntory Ltd.

• Yonekura-Sakakibara Keiko

  Plant Science Center, RIKEN (The Institute of Physical and Chemical Research

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