Gene Cloning and Overexpression of a Geranylgeranyl Diphosphate Synthase of an Extremely Thermophilic Bacterium, Thermus thermophilus

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概要

• 論文の詳細を見る

• A geranylgeranyl diphosphate (GGPP) synthase gene of an extremely thermophilic bacterium, Thermus thermophilus, was cloned and sequenced. T. thermophilus GGPP synthase, overexpressed in Escherichia coli cells as a glutathione S-transferase fusion protein, was purified and characterized. The fusion protein, retaining thermostability, formed a homodimer, and showed higher specific activity than did a partially purified thermostable enzyme previously reported. Optimal reaction conditions and kinetic parameters were also examined. The deduced amino acid sequence indicated that T. thermophilus GGPP synthase was excluded from the group of bacterial type GGPP synthases and lacked the insertion amino acid residues in the first aspartate-rich motif as do archaeal and eukaryotic shortchain prenyltransferases.

• 社団法人日本農芸化学会の論文
• 1999-02-23

著者

• Nishino Tokuzo

  Department of Biomolecular Engineering, Graduate School of Engineering, Tohoku University

• Muramatsu Masayoshi

  Bio Research Lab., Toyota Motor Corporation

• Ohto Chikara

  Bio Research Lab., Toyota Motor Corporation

• Obata Shusei

  Bio Research Lab., Toyota Motor Corporation

• 西野 朋子

  Department Of Biochemistry And Department Of Molecular Biology Nippon Medical School

• Nishino Takeshi

  日本医科大学 医科生物科学

• Nishino T

  Department Of Biochemistry And Engineering Faculty Of Engineering Tohoku University

• Nishino Tokuzo

  Dep. Of Biochemistry And Engineering Fac. Of Engineering Tohoku Univ.

• Obata Shusei

  Bio Research Lab. Toyota Motor Corporation

• Obata Shusei

  Bio Res. Lab. Toyota Motor Corp. 1 Toyota-cho Toyota 471-8572 Jpn

• Ohto Chikara

  Bio Research Lab. Toyota Motor Corporation

• Ohto Chikara

  Bio Res. Lab. Toyota Motor Corp. 1 Toyota-cho Toyota 471-8572 Jpn

• ISHIDA Chika

  Bio Research Laboratory, Toyota Motor Corporation

• KOIKE-TAKESHITA Ayumi

  Bio Research Laboratory, Toyota Motor Corporation

• YOKOYAMA Ken

  Bio Research Laboratory, Toyota Motor Corporation

• KOIKE-TAKESHITA Ayumi

  Department of Chemistry, Tokyo Institute of Technology

• Nishino Takeshi

  Department Of Microbiology Kyoto Pharmaceutical University

• Koike-takeshita Ayumi

  Department Of Chemistry Tokyo Institute Of Technology

• Koike‐takeshita A

  Bio Research Laboratory Toyota Motor Corporation

• Koike-takeshita Ayumi

  Bio Research Laboratory Toyota Motor Corporation

• Ishida Chika

  Bio Research Laboratory Toyota Motor Corporation

• Nishino Tokuzo

  Department Of Biochemistry And Engineering Faculty Of Engineering Tohoku University

• Ohnuma S

  Bio Research Lab. Toyota Motor Corporation

• Muramatsu M

  Bio Research Laboratory Toyota Motor Corporation

• Yokoyama Ken

  Bio Research Laboratory Toyota Motor Corporation:(present Address)department Of Biochemistry Faculty

• Muramatsu Masayoshi

  Bio Research Laboratory, Toyota Motor Corporation

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