Thermostable Collagenolytic Activity of a Novel Thermophilic Isolate, Bacillus sp.Strain NTAP-1
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概要
- 論文の詳細を見る
We isolated an acidophilic thermophile belonging to the genus Bacillus, strain NTAP-1, which secreted a thermostable collagenolytic activity into the culture medium. The collagenolytic activity exhibited an optimum pH for Azocoll hydrolysis of pH 3.9 and was not completely inhibited by 10 mM ethylenediaminetetraacetic acid(residual activity, 63%), suggesting that Bacillus NTAP-1 produces a novel acid proteinase with highest activity for collagen. The collagenolytic activity was thermostable; more than 80% of the original activity was retained after incubation of the culture supernatant at pH 4.0 and 60℃ for 4 h.
- 社団法人日本生物工学会の論文
- 2000-06-25
著者
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Nishino Tokuzo
Department of Biomolecular Engineering, Graduate School of Engineering, Tohoku University
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Nakayama Toru
Department of Biomolecular Engineering, Graduate School of Engineering, Tohoku University
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Nishino Tokuzo
Department Of Biomolecular Engineering Graduate School Of Engineering Tohoku University
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Nakayama Toru
Department Of Biomolecular Engineering Graduate School Of Engineering Tohoku University
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Tsuruoka Naoki
Department of Biochemistry, Kanazawa Medical University School of Medicine
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Tsuruoka Naoki
Department Of Biomolecular Engineering Graduate School Of Engineering Tohoku University
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Nishino Tokuzo
Department Of Biochemistry And Engineering Faculty Of Engineering Tohoku University
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AKAI MINORU
Department of Biomolecular Engineering, Graduate School of Engineering, Tohoku University
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Tsuruoka Naoki
Department Of Biochemistry Kanazawa Medical University School Of Medicine
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Akai Minoru
Department Of Biomolecular Engineering Graduate School Of Engineering Tohoku University
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NAKAYAMA Toru
Department of Applied Microbial Technology, The Kumamoto Institute of Technology:(Present office)Institute for Fundamental Research, Suntory Ltd.
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