Anthocyanin acyltransferase engineered for the synthesis of a novel polyacylated anthocyanin
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概要
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Some polyacylated anthocyanins, anthocyanins containing two or more aromatic acyl groups, are blue in color within a wide range of pH values, including acidic and neutral conditions even in the absence of any co-pigments or metal ions. We engineered a mutant of a malonyl-CoA:anthocyanin 5-O-glucoside-6″′-O-malonyltransferase of Salvia splendens (Ss5MaT1), Ss5AT306, which has an acquired specificity for hydroxycinnamoyl-CoA and is able to produce novel polyacylated anthocyanins. Ss5AT306 showed 6″′-O-hydroxycinnamoyltransferase activity toward some anthocyanins in addition to the 6″′-O-malonyltransferase activity retained (relative activities for acyl transfer to shisonin: malonyl-CoA, 100%; p-coumaroyl-CoA, 132%; caffeoyl-CoA, 103%). This alteration of acyl-donor specificity was achieved by the substitutions of only three contiguous amino acid residues, Val39-Arg40-Arg41, to the corresponding residues of anthocyanin aromatic acyltransferases, Met-Leu-Gln, suggesting that these amino acid residues are key residues governing the malonyl-CoA specificity of Ss5MaT1. Through the use of Ss5AT306, a novel polyacylated anthocyanin, p-coumaroylshisonin, with one aromatic acyl group in each of the 3-O-glucosyl and 5-O-glucosyl moieties of its structure, was produced and characterized in vitro. p-Coumaroylshisonin was bluer in color and displayed stronger color intensity than did shisonin, implying that accumulation of polyacylated anthocyanins such as p-coumaroylshisonin cause the modulation of flower colors.
- 日本植物細胞分子生物学会の論文
- 2007-12-01
著者
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Nishino Tokuzo
Department of Biomolecular Engineering, Graduate School of Engineering, Tohoku University
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Nakayama Toru
Department of Biomolecular Engineering, Graduate School of Engineering, Tohoku University
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Nishino Tokuzo
Department Of Biomolecular Engineering Graduate School Of Engineering Tohoku University
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Nishino Tokuzo
Tohoku Univ. Sendai Jpn
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Nishino Tokuzo
Dep. Of Biochemistry And Engineering Graduate School Of Engineering Tohoku Univ. Aoba-yama Sendai Mi
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Nakayama Toru
Department Of Biomolecular Engineering Graduate School Of Engineering Tohoku University
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SUZUKI Hirokazu
Department of Medicinal Chemistry, Faculty of Pharmaceutical Sciences, Hokuriku University
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Suzuki Hirokazu
Department Of Biomolecular Engineering Graduate School Of Engineering Tohoku University
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Nishino Tokuzo
Department Of Biochemistry And Engineering Faculty Of Engineering Tohoku University
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Suzuki Hirokazu
Department Of Applied Chemistry College Of Engineering Chubu University
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NAKAYAMA Toru
Department of Applied Microbial Technology, The Kumamoto Institute of Technology:(Present office)Institute for Fundamental Research, Suntory Ltd.
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