Overexpression of an Archaeal Geranylgeranyl Diphosphate Synthase in Escherichia coli Cells
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概要
- 論文の詳細を見る
An archaeal geranylgeranyl diphosphate synthase was overexpressed in Escherichia coli cells as fusion proteins. These fusion proteins retained their thermostability and had higher specific activity than did a partially purified native enzyme Previously reported. We purified 24.3 mg of MBP (maltose-binding protein)-fusion protein from a one-liter culture of E. coli. The MBP-fusion proteins existed in dimer, tetramer, octamer, or dodecamer form, and their product specificities were altered according to the oligomerization. The MBP-fusion protein has protease-sensitive sites in the portion corresponding to geranylgeranyl diphosphate synthase.
- 社団法人日本農芸化学会の論文
- 1998-06-23
著者
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Nishino Tokuzo
Department of Biomolecular Engineering, Graduate School of Engineering, Tohoku University
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Ohto Chikara
Bio Research Lab., Toyota Motor Corporation
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Obata Shusei
Bio Research Lab., Toyota Motor Corporation
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西野 朋子
Department Of Biochemistry And Department Of Molecular Biology Nippon Medical School
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Nishino Takeshi
日本医科大学 医科生物科学
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Nishino T
Department Of Biochemistry And Engineering Faculty Of Engineering Tohoku University
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Nishino Tokuzo
Dep. Of Biochemistry And Engineering Fac. Of Engineering Tohoku Univ.
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Obata Shusei
Bio Research Lab. Toyota Motor Corporation
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Obata Shusei
Bio Res. Lab. Toyota Motor Corp. 1 Toyota-cho Toyota 471-8572 Jpn
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Ohto Chikara
Bio Research Lab. Toyota Motor Corporation
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Ohto Chikara
Bio Res. Lab. Toyota Motor Corp. 1 Toyota-cho Toyota 471-8572 Jpn
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Hemmi H
Tohoku Univ. Miyagi Jpn
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Hemmi Hisashi
Department Of Biomolecular Engineering Graduate School Of Engineering Tohoku University
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Hemmi Hisashi
Department Of Biochemistry And Engineering Tohoku University
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Hemmi Hisashi
Dep. Of Biochemistry And Engineering Graduate School Of Engineering Tohoku Univ. Aoba-yama Sendai Mi
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NAKANE Hiroyuki
Bio Research Laboratory. Toyota Motor Corporation
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OHNUMA Shinichi
Department of Biochemistry and Engineering, Tohoku University
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Hamada Hiroaki
Department Of Life Science Faculty Of Science Okayama University Of Science
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Nishino Tokuzo
Department Of Biochemistry And Engineering Faculty Of Engineering Tohoku University
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Ohnuma S
Bio Research Lab. Toyota Motor Corporation
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Nakane Hiroyuki
Department Of Physics Graduate School Of Science Nagoya University
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Hemmi Hisashi
Department Of Applied Molecular Biosciences Graduate School Of Bioagricultural Sciences Nagoya Unive
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