Bacillus subtilis Spore Coat Protein LipC Is a Phospholipase B
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概要
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In Bacillus subtilis, the germination-related lipase LipC is located in the spore coat, and mutant spores are defective in L-alanine-stimulated germination. To determine the physiological role of LipC, the recombinant LipC expressed in Escherichia coli was purified and characterized. The enzyme hydrolyzes p-nitrophenyl ester substrates with various acyl-chain lengths. Thin-layer chromatography and gas chromatography-mass spectrometry analysis indicated that LipC cleaves the fatty acids at the sn-1 and sn-2 positions of phospholipids as phospholipase B, and that the enzyme shows no selectivity for the polar head groups of lipid molecules. When the amounts of free fatty acids in dormant wild-type and lipC mutant (YCSKd) spores were measured, the amount of free fatty acids in the YCSKd spores was about 35% less than in the wild-type spores. These results suggest the possibility that Bacillus subtilis LipC plays an important role in the degradation of the outer spore membrane during sporulation.
- 2010-01-23
著者
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Hemmi Hisashi
Department Of Biomolecular Engineering Graduate School Of Engineering Tohoku University
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Hemmi Hisashi
Department Of Biochemistry And Engineering Tohoku University
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Hemmi Hisashi
Dep. Of Applied Molecular Biosciences Graduate School Of Bioagricultural Sciences Nagoya Univ.
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Kato Shiro
Department of Applied Molecular Biosciences, Graduate School of Bioagricultural Sciences, Nagoya Uni
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Yoshimura Tohru
Department of Applied Molecular Biosciences, Graduate School of Bioagricultural Sciences, Nagoya Uni
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MORIYAMA Ryuichi
Department of Food and Nutritional Sciences, College of Bioscience and Biotechnology, Chubu Universi
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Masayama Atsushi
Department of Applied Molecular Biosciences, Graduate School of Bioagricultural Sciences, Nagoya Uni
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Terashima Takuya
Department of Applied Molecular Biosciences, Graduate School of Bioagricultural Sciences, Nagoya Uni
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Yoshimura Tohru
Dep. Of Applied Molecular Biosciences Graduate School Of Bioagricultural Sciences Nagoya Univ.
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Moriyama Ryuichi
Dep. Of Food And Nutritional Sciences Coll. Of Bioscience And Biotechnology Chubu Univ.
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Moriyama Ryuichi
Department Of Applied Biological Science School Of Agriculture Nagoya University
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Kato Shiro
Department Of Applied Molecular Biosciences Graduate School Of Bioagricultural Sciences Nagoya Unive
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Yoshimura Tohru
Department Of Applied Molecular Biosciences Graduate School Of Bioagricultural Sciences Nagoya Unive
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MOLGAARD Anne
Department of Chemistry, University of Copenhagen
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Masayama Atsushi
Department Of Applied Molecular Biosciences Graduate School Of Bioagricultural Sciences Nagoya Unive
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Terashima Takuya
Department Of Applied Molecular Biosciences Graduate School Of Bioagricultural Sciences Nagoya Unive
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Molgaard Anne
Department Of Chemistry University Of Copenhagen
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Hemmi Hisashi
Department Of Applied Molecular Biosciences Graduate School Of Bioagricultural Sciences Nagoya Unive
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