Mode of Action of a Germination-Specific Cortex-Lytic Enzyme, SleC, of Clostridium perfringens S40
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概要
- 論文の詳細を見る
The hydrolysis of the bacterial spore peptidoglycan (cortex) is a crucial event in spore germination. It has been suggested that SleC and SleM, which are conserved among clostridia, are to be considered putative cortex-lytic enzymes in Clostridium perfringens. However, little is known about the details of the hydrolytic process by these enzymes during germination, except that SleM functions as a muramidase. Muropeptides derived from SleC-digested decoated spores of a Bacillus subtilis mutant that lacks the enzymes, SleB, YaaH and CwlJ, related to cortex hydrolysis were identified by amino acid analysis and mass spectrometry. The results suggest that SleC is most likely a bifunctional enzyme possessing lytic transglycosylase activity and N-acetylmuramoyl-L-alanine amidase activity confined to cross-linked tetrapeptide-tetrapeptide moieties of the cortex structure. Furthermore, it appears that during germination of Clostridium perfringens spores, SleC causes merely small and local changes in the cortex structure, which are necessary before SleM can function.
- 社団法人 日本農芸化学会の論文
- 2007-04-23
著者
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Yoshimura Tohru
Department of Applied Molecular Biosciences, Graduate School of Bioagricultural Sciences, Nagoya Uni
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MORIYAMA Ryuichi
Department of Food and Nutritional Sciences, College of Bioscience and Biotechnology, Chubu Universi
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Masayama Atsushi
Department of Applied Molecular Biosciences, Graduate School of Bioagricultural Sciences, Nagoya Uni
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MAKINO Shio
Department of Applied Molecular Biosciences, Graduate School of Bioagricultural Sciences, Nagoya Uni
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Kumazawa Toshihiko
Department of Applied Molecular Biosciences, Graduate School of Bioagricultural Sciences, Nagoya Uni
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Fukuoka Satoshi
National Institute of Advanced Industrial Science and Technology (AIST)
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Makino Shio
Dep. Of Applied Molecular Biosciences Graduate School Of Bioagricultural Sciences Nagoya Univ.
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Makino Shio
Department Of Applied Biological Science Faculty Of Agriculture Nagoya University
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Makino S
Nagoya Univ. Aichi Jpn
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Yoshimura Tohru
Dep. Of Applied Molecular Biosciences Graduate School Of Bioagricultural Sciences Nagoya Univ.
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Moriyama Ryuichi
Dep. Of Food And Nutritional Sciences Coll. Of Bioscience And Biotechnology Chubu Univ.
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Moriyama Ryuichi
Department Of Applied Biological Science School Of Agriculture Nagoya University
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Yoshimura Tohru
Department Of Applied Molecular Biosciences Graduate School Of Bioagricultural Sciences Nagoya Unive
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Kumazawa Toshihiko
Department Of Applied Molecular Biosciences Graduate School Of Bioagricultural Sciences Nagoya Unive
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Masayama Atsushi
Department Of Applied Molecular Biosciences Graduate School Of Bioagricultural Sciences Nagoya Unive
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