Expression of Germination-Related Enzymes, CspA, CspB, CspC, SleC, and SleM, of Clostridium perfringens S40 in the Mother Cell Compartment of Sporulating Cells
スポンサーリンク
概要
- 論文の詳細を見る
In Clostridium perfringens S40, spore germination-specific enzymes are synthesized during sporulation. Previous reports have demonstrated that two cortex-lytic enzymes, SleC and SleM, and a component of germination-specific protease, CspC, are located outside the cortex as an integral part of the dormant spore. In the present study, we examined the time and compartment of these enzymes’ gene expression using reverse transcription-PCR (RT-PCR) and fluorescence microscopy on green fluorescence protein (GFP)-fused proteins. These results suggested that CspABC, SleC, and SleM are synthesized in the mother cell compartment of sporulating cells, probably at stages II ~ III of sporulation, and that the expression of cspABC genes is tricistronic.
- 日本遺伝学会の論文
- 2006-08-25
著者
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MORIYAMA Michie
Department of Home Economics, Aichi Gakusen University
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Kato Shiro
Department of Applied Molecular Biosciences, Graduate School of Bioagricultural Sciences, Nagoya Uni
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Yoshimura Tohru
Department of Applied Molecular Biosciences, Graduate School of Bioagricultural Sciences, Nagoya Uni
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MORIYAMA Ryuichi
Department of Food and Nutritional Sciences, College of Bioscience and Biotechnology, Chubu Universi
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Masayama Atsushi
Department of Applied Molecular Biosciences, Graduate School of Bioagricultural Sciences, Nagoya Uni
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HAMASAKI Keishi
Department of Applied Molecular Biosciences, Graduate School of Bioagricultural Sciences, Nagoya Uni
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URAKAMI Kenji
Department of Applied Molecular Biosciences, Graduate School of Bioagricultural Sciences, Nagoya Uni
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SHIMAMOTO Seiko
Department of Applied Molecular Biosciences, Graduate School of Bioagricultural Sciences, Nagoya Uni
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MAKINO Shio
Department of Applied Molecular Biosciences, Graduate School of Bioagricultural Sciences, Nagoya Uni
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Makino Shio
Dep. Of Applied Molecular Biosciences Graduate School Of Bioagricultural Sciences Nagoya Univ.
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Makino Shio
Department Of Applied Biological Science Faculty Of Agriculture Nagoya University
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Urakami Kenji
Department Of Applied Molecular Biosciences Graduate School Of Bioagricultural Sciences Nagoya Unive
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Urakami Kenji
Department Of Applied Biological Sciences School Of Agricultural Sciences Nagoya University
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Makino S
Nagoya Univ. Aichi Jpn
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Yoshimura Tohru
Dep. Of Applied Molecular Biosciences Graduate School Of Bioagricultural Sciences Nagoya Univ.
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Shimamoto Seiko
Department Of Applied Molecular Biosciences Graduate School Of Bioagricultural Sciences Nagoya Unive
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Hamasaki Keishi
Department Of Applied Molecular Biosciences Graduate School Of Bioagricultural Sciences Nagoya Unive
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Moriyama Michie
Department Of Home Economics Aichi Gakusen College
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Moriyama Ryuichi
Dep. Of Food And Nutritional Sciences Coll. Of Bioscience And Biotechnology Chubu Univ.
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Moriyama Ryuichi
Department Of Applied Biological Science School Of Agriculture Nagoya University
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Kato Shiro
Department Of Applied Molecular Biosciences Graduate School Of Bioagricultural Sciences Nagoya Unive
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Yoshimura Tohru
Department Of Applied Molecular Biosciences Graduate School Of Bioagricultural Sciences Nagoya Unive
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Masayama Atsushi
Department Of Applied Molecular Biosciences Graduate School Of Bioagricultural Sciences Nagoya Unive
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