Subcellular Localization of a Germiantion-specific Cortex-lytic Enzyme, SleB, of Bacilli during Sporulation
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概要
- 論文の詳細を見る
The subcellular localization of a germination-specific cortex-lytic enzyme, SleB, of Bacillus subtilis during sporulation was observed by using fusions of N-terminal region of SleB to the green fluorescent protein (GFP). A fusion with a putative peptidoglycan-binding motif (SleB1-108-GFP) formed a fluorescent ring around the forespore of the wild type strain, as expected from the known location of the intact SleB in the dormant spore. SleB1-108-GFP formed a similar fluorescent ring around the forespore of the gerE mutant which has a severe defect in the coat structure, and of the cwlD mutant which lacks a muramic δ-lactam unique to the spore peptidoglycan (cortex), whereas the fusion could not attach to the spore of the cwlDgerE mutant. By contrast, a fusion without the motif (SleB1-45-GFP) could not be recruited around the forespore of the gerE mutant though it appeared to be accumulated on the outside of the spore of the wild type strain. Since SleB was shown to degrade only the cortex with muramic δ-lactam, these results suggested that a proper localization of SleB requires a strict interaction between the motif of the enzyme and the δ-lactam structure of the cortex, not the formation of normal coat layer.
- 日本遺伝学会の論文
- 2006-06-25
著者
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MORIYAMA Michie
Department of Home Economics, Aichi Gakusen University
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Kato Shiro
Department of Applied Molecular Biosciences, Graduate School of Bioagricultural Sciences, Nagoya Uni
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Yoshimura Tohru
Department of Applied Molecular Biosciences, Graduate School of Bioagricultural Sciences, Nagoya Uni
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MORIYAMA Ryuichi
Department of Food and Nutritional Sciences, College of Bioscience and Biotechnology, Chubu Universi
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Masayama Atsushi
Department of Applied Molecular Biosciences, Graduate School of Bioagricultural Sciences, Nagoya Uni
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FUKUOKA Hideyuki
Department of Applied Molecular Biosciences, Graduate School of Bioagricultural Sciences, Nagoya Uni
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Yoshimura Tohru
Dep. Of Applied Molecular Biosciences Graduate School Of Bioagricultural Sciences Nagoya Univ.
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Moriyama Michie
Department Of Home Economics Aichi Gakusen College
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Fukuoka Hideyuki
Department Of Applied Molecular Biosciences Graduate School Of Bioagricultural Sciences Nagoya Unive
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Moriyama Ryuichi
Dep. Of Food And Nutritional Sciences Coll. Of Bioscience And Biotechnology Chubu Univ.
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Moriyama Ryuichi
Department Of Applied Biological Science School Of Agriculture Nagoya University
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Kato Shiro
Department Of Applied Molecular Biosciences Graduate School Of Bioagricultural Sciences Nagoya Unive
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Yoshimura Tohru
Department Of Applied Molecular Biosciences Graduate School Of Bioagricultural Sciences Nagoya Unive
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Masayama Atsushi
Department Of Applied Molecular Biosciences Graduate School Of Bioagricultural Sciences Nagoya Unive
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