USE OF BIOTINYLATED ANTIBODY FOR THE ASSAY OF HANGANUTZIU-DEICHER ANTIBODIES AND ANTIGENS IN FLUIDS AND TISSUES FROM CANCER PATIENTS
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概要
- 論文の詳細を見る
An improved enzyme-linked immunosorbent assay (ELISA) for detection of heterophile Hanganutziu-Deicher (HD) antibodies and antigens, which are frequently detected in sera and/or cancerous tissues from patients with various cancers was developed using biotinylated chicken anti-GM3(NeuGc) antibody and avidin-horse raddish peroxidase conjugate. The N-glycolylneuraminyllactosyl-ceramide, GM3(NeuGc) ganglioside was purified from horse erythrocyte memberanes. The ELISA proceduure required 300 ng GM3(NeuGc) antigen to coat plastic microtiter plates and 190 ng biotinylated antibody per well to give optimum product formation. The technique could detect 6 ng antigen in tissue homogenate as compared to 0.6 ng of the pure compound by inhibition. Chicken anti-GM3(NeuGc) antibody quantitatively inhibited the biotinylated antibody, however, this procedure was not suitable to quantify lower affinity HD antibody in patient sera. Immunostaining specific for HD antigen-positive cells, in tissue sections was by 4 μg/ml biotinylted antibody and 200 dilution of Avidin-biotinylated peroxidase complex reagent using pig intestine and lymph node as positive tissues and chicken intestine and lung as negative tissues.
- 北海道大学の論文
- 1989-06-20
著者
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Kato Shiro
Department of Applied Molecular Biosciences, Graduate School of Bioagricultural Sciences, Nagoya Uni
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Naiki M
National Inst. Health Tokyo Jpn
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NAIKI Masaharu
Department of Veterinary Science, National Institute of Health
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GATHURU John
Department of Biochemistry, Faculty of Veterinary Medicine
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Kato S
Department Of Pathology Research Institute For Microbial Diseases Osaka University
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Usuba Osamu
Department Of Parasitology Yamagata University
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Gathuru John
Department Of Biochemistry Faculty Of Veterinary Medicine Hokkaido University
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Naiki M
Department Of Veterinary Science
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Naiki Masaharu
Department Of Biochemistry Faculty Of Veterinary Medicine Hokkaido University:(present Address) Depa
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Kato Shiro
Department Of Applied Molecular Biosciences Graduate School Of Bioagricultural Sciences Nagoya Unive
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HIGASHI Hideyoshi
Department of Pathology, Research Institute for Microbial Diseases, Osaka University
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Higashi Hideyoshi
Department Of Pathology Research Institute For Microbial Diseases Osaka University
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Higashi Hideyoshi
Department Of Biochemistry Faculty Of Veterinary Medicine Hokkaido University
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