THE NUCLEOTIDE SEQUENCE OF THE GENES, fanE AND fanF, OF Escherichia coli K99 FIMBRIAE
スポンサーリンク
概要
- 論文の詳細を見る
K99 fimbriae of enterotoxigenic Escherichia coli consist of eight different subunits. A major subunit called fimbrillin forms fimbrial structure and a minor subunit called adhesin localizes at the tip of fimbriae and recognizes host receptor ganglioside. Within this eight gene cluster, fanE and fanF have not yet been sequenced. In this study, fanE and fanF genes were sequenced by analyzing several DNA fragments produced by endonuclease or exonuclease digestion. The fanE gene encoded 227 amino acids containing 20 amino acids of signal peptide starting from GTG (valine) and showed a homology to fanA-fanB. The fanF gene encoded 271 amino acids containing 20 amino acids of signal peptide starting from ATG (methionine) and showed homologies to the fanD gene, fimbrillin gene of F41,adhesin gene of P fimbriae (papG) and adhesin gene of Type 1 fimbriae (fimH). E and F subunits had fifteen and fourteen hydrophobic domains, respectively, which periodically appeared possibly forming a hydrophobic region.
- 北海道大学の論文
- 1991-05-30
著者
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NAIKI Masaharu
Department of Veterinary Science, National Institute of Health
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ONO Eriko
Department of Microbiology, Hoshi College of Pharmacy
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Ono Eriko
Department Of Biochemistry Faculty Of Veterinary Medicine Hokkaido University
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Lavin Martin
Queensland Inst. Med. Res.
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Lavin Martin
Queensland Institute Of Medical Research
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Naiki Masaharu
Department Of Biochemistry Faculty Of Veterinary Medicine Hokkaido University:(present Address) Depa
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Ono E
Toho Univ. Chiba Jpn
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