Stimulation of in Vitro Insulin Action by Glycinin Acidic Subunit A_<1a>(Biological Chemistry)

概要

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We have reported that the trypsinized product of glycinin acidic subunit A_<1a> (A_<1a>/Tr) modulates insulin action on fat decomposition in vitro [Minami et al., Agric. Biol. Chem., 54, 511 (1990)]. This study was undertaken to get additional information on the modulation of insulin action by A_<1a>/Tr. A_<1a>/Tr shifted the insulin-response curve of isoproterenol-stimulated lipolysis in isolated rat adipocytes to a lower insulin concentration and increased the maximal response elicited by the hormone. However, A_<1a>/Tr had neither an effect on the ability of insulin to stimulate glucose transport nor on the insulin binding to its receptor. Thus, A_<1a>/Tr potentiated some level(s) of insulin actions in vitro. A characteristic feature was that A_<1a>/Tr suppressed degradation of insulin that was internalized in a receptor-mediated manner. This protective effect alone, however, could not explain the total effects of A_<1a>/Tr. A possible explanation is offered for the mechanism of A_<1a>/Tr action. Apart from the action of A_<1a>/Tr on insulin, our results indicate a dissociation of the effect of insulin on glucose transport from that on lipolysis.
社団法人日本農芸化学会の論文
1991-04-23