A STAINING PROCEDURE FOR NERVE FIBERS IN WHOLE MOUNT PREPARATIONS OF THE MEDAKA AND CHICK EMBRYOS

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概要

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• A simple and reproducible technique is described by which the nerve fibers in small fish and chick embryos are revealed in whole mounts. The materials were fixed, washed, pre-treated by various procedures and finally stained immunohistochemically by using an antibody directed against neurofilament protein. The cranial, spinal and sympathetic nerve fibers were stained. In the medaka, pit organs were also stained. Moreover, it was possible to stain the nerve fibers in the same material repeatedly. By this method, the three dimensional structures of the nerve fibers and their relationships to other structures can be readily observed.

• 日本組織細胞化学会の論文

著者

• TANAKA SHIGENORI

  Department of Anatomy and Neuroembryology, Kanazawa University

• Tanaka S

  Shizuoka Univ. Shizuoka Jpn

• Tanaka S

  Department Of Biology Faculty Of Science Shizulka University

• Kuratani Shigeru

  Lab. for Evol. Morph, RIKEN CDB

• Zukeran Chosei

  Department Of Anatomy Faculty Of Medicine University Of The Ryukyus

• Kuratani S

  Lab. For Evol. Morph Riken Cdb

• Kuratani Shigeru

  Department Of Anatomy Faculty Of Medicine University Of The Ryukyus

• Ishikawa Yuji

  Department Of Anatomy Faculty Of Medicine University Of The Ryukyus

• Tanaka Shigenori

  Department Of Anatomy And Neuroembryology Graduate School Of Medical Science Kanazawa University

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