An Anti-Peptide Antibody that Recognized Unexpected Protein-A Case Report
スポンサーリンク
概要
- 論文の詳細を見る
Rabbits were immunized with partial peptides corresponding to dog Na^+-coupled myoinositol cotransporter(SMIT)and rat water channel aquaporin 3(AQP3).Immunoblot analysis and immunofluorescence microscopy revealed that the antibodies corresponding to SMIT and to AQP3 both detected AQP3 protein in the kidney. Neither of them, on the other hand, detected SMIT protein. Immunogen peptides of both SMIT and AQP3 contain the identical sequence "KEQI", whereas other amino acids are different from each other. Immunohistochemical preabsorption studies demonstrated that the antibodies corresponding to SMIT mainly recognized the sequence "KEQI" as an epitope. These observations demonstrate that the utmost care should be taken in using anti-peptide antibodies.
- 日本組織細胞化学会の論文
著者
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Suzuki T
Department Of Anatomy And Cell Biology Gunma University Graduate School Of Medicine
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Tanaka Shigeyasu
Department Of Biology Faculty Of Science Shizuoka University
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Tanaka S
Shizuoka Univ. Shizuoka Jpn
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Tanaka S
Department Of Biology Faculty Of Science Shizulka University
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Takata Kuniaki
Department Of Anatomy And Cell Biology Gunma University Graduate School Of Medicine
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Suzuki Takeshi
Laboratory Of Soil Science Faculty Of Agriculture Kobe University
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Matsuzaki Toshiyuki
Laboratory of Molecular and Cellular Morphology, Institute for Molecular and Cellular Regulation, Gu
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Takata Kuniaki
Laboratory Of Molecular And Cellular Morphology Institute For Molecular And Cellular Regulation Gunm
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Takata Kuniaki
Laboratory Of Molecular And Cellular Morphology Gunma University
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Matsuzaki Toshiyuki
Department Of Anatomy And Cell Biology Gunma University Graduate School Of Medicine
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Suzuki Takeshi
Laboratory Of Microbial Biochemistry Institute For Chemical Research Kyoto University
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TANAKA Shigeyasu
Department of Anatomy, Josai Dental College
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