Seasonal Variation in Pituitary Gonadotropin in the Adult Male Newt, <I>Cynops Pyrrhogaster Pyrrhogaster</I>, Revealed by Isoelectric Focusing Technique and Radioreceptor Assay
スポンサーリンク
概要
- 論文の詳細を見る
The seasonal variation in pituitary gonadotropin in the adult male newt, <I>Cynops pyrrhogaster pyrrhogaster</I> was investigated by means of isoelectric focusing (IEF) coupled with radioreceptor assay (RRA), which employed <I>Anolis</I> or <I>Xenopus</I> testicular homogenates as receptors and <SUP>125</SUP>I-rat FSH as radioligand. In the Anolis RRA system, the standard curve was obtained with 0.125-16 ng/tube of NIAMDD rat FSH I-3. Purified preparations, chicken LH IEF-1, chicken FSH AGCHD11113A and bullfrog basic gonadotropin-IV competitively inhibited the binding of the radioligand, but NIAMDD rat LH I-4 and human chorionic gonadotropin did not crossreact. The autoradiographic study revealed that <SUP>125</SUP>I-rat FSH bound to the constituent cells of the seminiferous tubules in the <I>Anolis</I> testis, but scarcely to Leydig cells. In the IEF pattern of gonadotropin in February obtained by <I>Anolis</I> RRA, distinct peaks were observed at pH 9.05 (component B) and 8.55 (component C), and less distinct peaks were observed at pH 9.80 (component A), 7.55 (component D) and 7.05 (component E). When the same fractions were assayed by <I>Xenopus</I> RRA, five components were found in the alkaline region, which corresponded to those observed with <I>Anolis</I> RRA. Similar results were obtained with pituitary extracts in May. In July, the IEF pattern obtained by <I>Anolis</I> RRA indicated two additional components at pH 6.30 (component F) and 5.27 (component G) in the acidic region, which were not found by <I>Xenopus</I> RRA. The relationship between the testicular function and the nature of pituitary gonadotropin in the reproductive cycle was discussed.
- 社団法人 日本内分泌学会の論文
著者
-
Tanaka Shigeyasu
Department Of Biology Faculty Of Science Shizuoka University
-
Wakabayashi Katsumi
Hormone Assay Center Institute of Endocrinology Gunma University
-
Takikawa Hiroo
Department Of Pharmaceutical Chemistry Insitute Of Endocrinology Gunma University
-
Wakabayashi Katsumi
Hormone Assay Center Institute Of Endocrinology Gumma Univ.
-
TANAKA SHIGEYASU
Department of Pharmaceutical Chemistry, Institute of Endocrinology, Gunma University
関連論文
- REGULATION OF THE EXPRESSION OF THE PROHORMONE CONVERTASES, PC1 AND PC2, BY CORTICOSTERONE
- CHARACTERIZATION OF AN AQUAPORIN, AQP-H3BL, EXPESSED IN MELANOTROPHS IN THE BULLFROG PARS INTERMEDIA
- EXPRESSION OF A- AND E-SUBUNITS OF VACUOLAR TYPE H^+ -ATPASE IN THE ENDOLYMPHATIC SAC OF THE BULLFROG(Endocrinology,Abstracts of papers presented at the 74^ Annual Meeting of the Zoological Society of Japan)
- Molecular Cloning and Expression of Prohormone Convertases PC1 and PC2 in the Pituitary Gland of the Bullfrog, Rana catesbeiana(Endocrinology)
- GENE EXPRESSION OF THE PROHORMONE CONVERTASES PC1 AND PC2 IN THE ULTIMOBRANCHIAL GLAND OF THE BULLFROG
- Prohormone convertases (PC1/3 and PC2) in rat and human pancreas and islet cell tumors : Subcellular immunohistochemical analysis
- Analysis of Salmon Calcitonin I in the Ultimobranchial Gland and Gill Filaments during Development of Rainbow Trout, Oncorhynchus mykiss, by in situ Hybridization and Immunohistochemical Staining(Endocrinology)
- Transmission Electron Microscopy Study of the Microstructure in Selective-Area-Grown GaN and an AIGaN/GaN Heterostructure on a 7-Degree Off-Oriented (001) Si Substrate : Structure and Machanical and Thermal Properties of Condensed Matter
- POSSIBLE HORMONAL REGULATION ON DISSOLUTION OF CALCIUM CARBONATE CRYSTALS IN THE BULLFROG ENDOLYMPHATIC SAC
- Cloning and expression of vacuolar proton-pumping ATPase subunits in the follicular epithelium of the bullfrog endolymphatic sac
- ANALYSIS OF mRNAS EXPRESSED IN THE PARATHYROID GLAND OF THE BULLFROG
- Ultrastructure and function of the Golgi apparatus in peptide hormone-secreting cells of the anterior pituitary gland
- EXPRESSION REGULATION OF PROHORMONE CONVERTASES, PC1 AND PC2, BY CORTICOSTERONE(Endocrinology,Abstracts of papers presented at the 75^ Annual Meeting of the Zoological Society of Japan)
- EXPRESSION OF PROHORMONE CONVERTASES PC1 AND PC2 IN PROOPIOMELANOCORTIN CELLS IN THE RAT ANTERIOR PITUITARY GLANDS
- CLONING OF THE cDNAS ENCODING BONE MORPHOGENETIC PROTEINS FROM RAINBOW TROUT
- MOLECULAR HISTOLOGICAL ANALYSIS OF DEVELOPMENT OF THE THYROID GLAND IN THE RAINBOW TROUT
- EXPRESSION AND FUNCTION OF NKX2.1D (THYROID TRANSCRIPTION FACTOR-1D) IN THE ULTIMOBRANCHIAL GLAND OF THE RAINBOW TROUT(Endocrinology,Abstracts of papers presented at the 75^ Annual Meeting of the Zoological Society of Japan)
- DEVELOPMENT AND TRANSCRIPTION FACTORS OF THE ULTIMOBRANCHIAL GLAND OF RAINBOW TROUT
- CHANGES IN PLASMA TSH LEVELS DURING AND AFTER METAMORPHOSIS OF THE BULLFROG
- STIMULATION BY VARIOUS HYPOTHALAMIC FACTORS OF THE RELEASE OF IMMUNOASSAYABLE TSHβ FROM THE BULLFROG PITUITARY
- EFFECT OF ARGININE VASOTOCIN ON THE REPRODUCTIVE BEHAVIOR OF THE MALE NEWT, CYNOPS PYRRHOGASTER
- HORMONAL CONTROL OF THE REPRODUCTIVE BEHAVIOR OF THE REDBELLIED NEWT, CYNOPS PYRRHOGASTER
- EXPRESSION OF THE GLUCOCORTICOID RECEPTOR IN RAT IS INVOLVED IN CHANGES IN PROOPIOMELANOCORTIN PROCESSSING PATTERNS
- CLONING AND LOCALIZATION OF AQUAPORIN SPECIFICALLY EXPRESSED IN THE TOAD SKIN GLANDS(Endocrinology,Abstracts of papers presented at the 76^ Annual Meeting of the Zoological Society of Japan)
- PHYLOGENETIC STUDY ON ANURAN AQUAPORINS WITH A HIGH HOMOLOGY TO VASOTOCIN-DEPENDENT HYLA AQP-h3(Endocrinology,Abstracts of papers presented at the 76^ Annual Meeting of the Zoological Society of Japan)
- DYNAMICS OF VASOTOCIN-DEPENDENT ASUAPORINS IN THE VENTRAL SKIN OF THE TREE FROG (HYLA JAPONICA) IN RESPONSE TO HYDRINS INTERMEDIATES OF VASOTOCIN(Endocrinology,Abstracts of papers presented at the 76^ Annual Meeting of the Zoological Society of Japan)
- GENE EXPRESSION OF VASCULAR ENDOTHELIAL GROWTH FACTOR IN THE RAT PITUITARY DURING FORMATION OF THE VASCULAR SYSTEM IN THE HYPOTHALAMIC-PITUITARY AXIS(Endocrinology,Abstracts of papers presented at the 76^ Annual Meeting of the Zoological Society of Ja
- INVOLVEMENT OF VACUOLAR-H^+-ATPASE IN DISSOLVING BICARBONATE CRYSTALS LOCATED IN THE FOLLICLES OF THE ENDOLYMAPHIC SAC IN THE BULLFROG, RANA CATESBEIANA
- EFFECTS OF HYDRINS, INTERMEDIATES WHICH APPEAR DURING THE PROCESSING OF PROVASOTOCIN, ON WATER PERMEATION AND THE TRANSLOCATION OF AQUAPORINS IN THE PELVIC VENTRAL SKIN OF SEVERAL ANURAN AMPHIBIANS
- COMPARATIVE STUDIES ON THE PHOSPHORYLATION OF ANTIDIURETIC HORMONE-REGULATED AQUAPORIN IN THE ANURAN URINARY BLADDER
- ARGININE VASOTOCIN STIMULATES PHOSPHORYLATION OF AQUAPORIN-H2 IN THE FROG URINARY BLADDER(Endocrinology,Abstracts of papers presented at the 75^ Annual Meeting of the Zoological Society of Japan)
- POSSIBLE INVOLVEMENT OF BRAIN-DERIVED NEUROTROPHIC FACTOR (BDNF) IN THE INNERVATION OF DOPAMINERGIC NEURONS INTO THE RAT PARS INTERMEDIA(Endocrinology,Abstracts of papers presented at the 75^ Annual Meeting of the Zoological Society of Japan)
- SUBCELLULAR LOCALIZATION OF ANTIDIURETIC HORMONE-DEPENDENT AQUAPORIN, AQP-H2, IN THE URINARY BLADDER OF THE TREE FROGS, HYLA JAPONICA(Endocrinology,Abstracts of papers presented at the 74^ Annual Meeting of the Zoological Society of Japan)
- COMPARATIVE STUDY OF ANTIDIURETIC HORMONE-REGULATED AQUAPORIN IN ANURAN URINARY BLADDER : EXPRESSION AND LOCALIZATION OF AQP-H2 HOMOLOGUE
- Regulation of LH-Sensitive Adenylate Cyclase System by the Partical Agonists
- Immunohistochemical characterization of the intracellular pool of water channel aquaporin-2 in the rat kidney
- FORMATION AND DISSOLUTION OF CALCIUM CARBONATE CRYSTALS IN THE BULLFROG ENDOLYMPHATIC SAC
- IMMUNOCYTOCHEMICAL LOCALIZATION OF PROHORMONE CONVERTASES PC1 AND PC2 IN THE CALCITONIN/CGRP CELLS
- Mammalian Gonadotropins-Polymorphysm, Biosynthesis, and Release
- EXPRESSION OF α-MSH IN THE QUAIL BURSA OF FABRICIUS
- HORMONE-LIKE MOLECULES IN THE BURSA OF FABRICIUS
- IMMUNOFLUORESCENCE LOCALIZATION OF A WATER CHANNEL PROTEIN, AQP-h2, IN THE VASOTOCIN-DEPENDENT TISSUES OF THE TREE FROG, HYLA JAPONICA(Endocrinology)(Proceedings of the Seventy-Third Annual Meeting of the Zoological Society of Japan)
- ARGININE VASOTOCIN INDUCES TRANSLOCATION OF FROG AQUAPORINS, AQP-H2 AND AQP-H3, IN THE VENTRAL SKIN AND URINARY BLADDER
- DEVELOPMENTAL APPEARANCE OF A WATER CHANNEL, AQUAPORIN-H3, EXPRESSED SPECIFICALLY IN THE ABDOMINAL PELVIC SKINS OF THE JAPANESE TREE FROG, HYLA JAPONICA
- MOLECULAR DIVERSITY OF AQUAPORIN IN THE WATER ADAPTATION OF AMPHIBINIANS
- Raman Scattering of InGaAsP Lattice-Matched to GaAs in the Region of Immiseibility
- Analysis of Compositional Variation at Initial Transient Time in LPE Growth of InGaAsP/GaAs System
- Characterization of Interface Instability in InGaAsP LPE Growth on GaAs by Fourier Analysis
- Photochemical etching technique for preparing high-quality TEM samples of n-type compound semiconductors
- Amphibian and Reptilian Gonadotoropin : Biological Activity
- SYNTHESIS AND SECRETION OF PITUITARY GONADOTROPIN (GTH) FROM THE FROG, RANA JAPAONICA
- ADPITUITARY GLAND : FOLLICULAR STRUCTURE BEARING THYROTROPIN-IMMUNOREACTIVE CELLS ATTACHED TO THE CAUDAL REGION OF THE PARS DISTALIS IN THE FROG PITUITARY GRAND
- MOLECULAR CLONING OF ASCIDIAN PROHORMONE CONVERTASE : 2 AND PITUITARY HOMEOBOX (PITX) cDNAs
- MOLECULAR CLONING OF ASCIDIAN (HALOCYNTHIA RORETZI) PROHORMONE CONVERTASE 2 cDNA
- Comparative Aspects of Intracellular Proteolytic Processing of Peptide Hormone Precursors : Studies of Proopiomelanocortin Processing
- Distribution of Immunoreactive Thyrotropin-Releasing Hormone in the Brain and Hypophysis of Larval Bullfrogs with Special Reference to Nerve Fibers in the Pars Distalis : Endocrinology
- EXPRESSION AND LOCALIZATION OF Rab6 AND Rab12 IN THE SEMINIFEROUS TUBULES OF RAT TESTIS(Developmental Biology)(Proceedings of the Seventy-Third Annual Meeting of the Zoological Society of Japan)
- SPATIAL AND TEMPORAL EXPRESSION OF VASCULAR ENDOTHELIAL GROWTH FACTOR (VEGF) DURING THE FORMATION OF VASCULARIZATION IN THE HYPOTHALALAMIC-PITUITARY SYSTEM
- FUNCTIONAL RELATIONSHIP BETWEEN FROG VITELLOGENIN AND THE APPEARANCE OF TESTIS-OVA
- A WATER-CHANNEL AQUAPORIN IN THE AMPHIBIANS
- Absence of Methylated Thyrotropin-Releasing Hormone in the Bullfrog (Rana catesbeiana) Brain
- A-10 EFFECTS OF 1-METHYL-5-THIOTETRAZOLE ON SERUM AND PITUITARY FSH AND LH LEVELS IN RATS.
- The Presence of LH Components Having Different Ratios of Bioactivity to Immunoreactivity in the Rat Pituitary Glands
- Immunological and Biological Characteristics of a New TRF Analogue, L-Pyroglutamyl-L-Histidyl-L-Pipecolic Acid Amide
- A Possible Role of Cadaverine in the Biosynthesis of Polyamines in the Japanese Newt Testis
- Measurement of Human FSH by Radioreceptor Assay
- Species Specificity of Radioreceptor Assay and Radioimmunoassay for Rat FSH
- Effect of Gonadectomy and Steroid Treatment on the Receptor-Binding Activity and Immunoreactivity of Serum and Pituitary FSH in the Adult Rats of Both Sexes
- In vitro Interaction of Reproductive Steroids with Cellular Components of Cow Ovaries
- On the Presence of FSH-β Subunit in the Rat Anterior Pituitary and Serum
- An Anti-Peptide Antibody that Recognized Unexpected Protein-A Case Report
- Seasonal Variation in Pituitary Gonadotropin in the Adult Male Newt, Cynops Pyrrhogaster Pyrrhogaster, Revealed by Isoelectric Focusing Technique and Radioreceptor Assay
- Effects of X-Ray Irradiation on the Subsequent Gonadotropin Secretion in Normal and Neonatally Estrogenized Female Rats
- Heterogeneity of Rat Luteinizing Hormone Revealed by Radioimmunoassay and Electrofocusing Studies
- Different Profiles of Isoelectric Avian Luteinizing Hormone Components in Biological Activity and Immunoreactivity
- Radioimmunoassay. Problems of Measurement of Gonadotropins in Blood Samples
- THE ALTERATION OF ISOZYMIC PATTERNS IN LACTATE DEHYDROGENASE DURING ARTIFICIAL MATURATION OF RAT OVARY
- Seasonal Changes in Plasma Testosterone and 5α-Dihydrotestosterone Levels in the Adult Male Newt, Cynops Pyrrhogaster Pyrrhogaster
- Estrogen Binding Proteins in Ovarian Follicle
- SIMPLIFIED PROCEDURE FOR THE HISTOCHEMICAL DEMONSTRATION OF DEHYDROGENASE ACTIVITY IN RAT OVARIES
- Effect of AA560 (A Nonsteroidal Antiandrogen) Implantation In the Hypothalamus on Gonadotropin Secretion in Male Rats
- Action of a novel nonsteroidal antiandrogen, AA560.