Different Profiles of Isoelectric Avian Luteinizing Hormone Components in Biological Activity and Immunoreactivity
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概要
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Chicken LH components from the glycoprotein fraction of the anterior pituitary glands have been separated to isoelectric homogeneity by means of isoelectric focusing, and investigated for their biological activities <I>in vitro</I>. The activities of these components were measured with LH receptor binding, cyclic AMP accumulation and testosterone production in rat Leydig cells at equal doses expressed as immunoreactivity of IRC-2 (Gunma). All the components were active in the heterologous assay systems, although the relative potency expressed as the ratios of biological activity to immunoreactivity (B: I) differed significantly among the components. Component I, the amount of which is the largest (40-50%), with the most alkaline isoelectric point (pI) showed the highest B: I ratio, and the B: I ratio decreased with decreasing pI in the same way as in rat LH components (Wakabayashi, 1980; Hattori <I>et al</I>., 1983). Therefore, pituitary LH from photostimulated male quail, where the relative amount of component I was increased, was estimated to have higher B: I ratios than that from short-day (SD) treated male quail. Furthermore, the differences in activities among the components obtained by the three assays were in the following order: testosterone production>cyclic AMP accumulation>receptor binding, suggesting that the hormonal actions of components with higher B: I ratios (I, II and III) are efficiently amplified in the biological response to the final step. In the incubation of pituitary glands with hypothalamic extracts, component I in the pituitary glands from the long-day (LD) treated group was mostly decreased after the incubation, while all the components were decreased in parallel in the SD-treated group. The results suggest that the LH component releasable to GnRH changes in endocrine status though component I with the highest B: I ratio is relatively releasable in both SD-and LDtreated groups.
- 社団法人 日本内分泌学会の論文
著者
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Hattori Masa-aki
Hormone Assay Center Institute Of Endocrinology Gunma University
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Wakabayashi Katsumi
Hormone Assay Center Institute Of Endocrinology Gumma Univ.
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HATTORI MASA-AKI
Hormone Assay Center, Institute of Endocrinology, Gunma University
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Hattori Masaaki
Hormone Assay Center, Institute of Endocrinology, Gunma University
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