Measurement of Human FSH by Radioreceptor Assay
スポンサーリンク
概要
- 論文の詳細を見る
We established a sensitive RRA system for human FSH, employing PMS-treated immature rat ovary. The dissociation constant of the binding of the receptor preparation to NIAMDD human FSH-2 was 1.15×10<SUP>-10</SUP>M. The standard curve was obtained with 0.2-25.6ng/tube of NIAMDD hFSH-2. Purified hLH, hTSH, and HCG had no significant effect on the binding. When the anterior pituitary homogenates obtained from humans were assayed by this system, the intra-assay and inter-assay coefficients of variation were 11.9% and 13.4% respectively, and the assay values correlated well with those obtained by RIA. This assay is applicable for the measurement of FSH in serum, when the nonspecific inhibitor effects of serum are compensated for by the addition of merthiolate and when FSH-free serum is used instead of the buffer for the standard curve. The intraassay, and inter-assay coefficients of variation were 9.31% and 19.7% respectively. The assay values correlated with those obtained by RIA under the same physiological state. The ratio of the assay values RRA/RIA, was dependent upon the physiological state, e.g. 6.29 in men, 3.84 and 4.18 in women at follicular and luteal phase respectively and 2.40 in menopausal women. During the menstrual cycle, our results showed that the value of RRA/RIA derived from serum did not change significantly.
- 社団法人 日本内分泌学会の論文
著者
-
Wakabayashi Katsumi
Hormone Assay Center Institute of Endocrinology Gunma University
-
Igarashi Masao
Department Of Obstetrics And Gynecology School Of Medicine Gunma University
-
Minegishi Takashi
Department Of Gynecology And Reproductive Medicine Gunma University
-
Wakabayashi Katsumi
Hormone Assay Center Institute Of Endocrinology Gumma Univ.
-
IGARASHI MASAO
Department of Obsterics and Gynecology, Gunma University School of Medicine
関連論文
- Effects of Aging and Postmenopausal Hypoestrogenism on Skin Elasticity and Bone Mineral Density in Japanese Women
- Regulation of LH-Sensitive Adenylate Cyclase System by the Partical Agonists
- STIMULATORY AND INHIBITORY REGULATION OF GnRH ON THE PROLIFERATION OF CELLS WITH DIFFERENT LIGAND SENSITIVITIES
- Expression and Cyclin Change of Betaglycan in the Rat Oviduct
- Molecular Cloning, Cellular Distribution and Regulation of Rat Steroidogenic Acute Regulatory Protein (StAR) in the Ovary
- Mammalian Gonadotropins-Polymorphysm, Biosynthesis, and Release
- A-10 EFFECTS OF 1-METHYL-5-THIOTETRAZOLE ON SERUM AND PITUITARY FSH AND LH LEVELS IN RATS.
- Regulation of human luteinizing hormone receptor in the ovary
- Avian Gonadotropin-Releasing Hormones
- Relationships between circulating leptin concentrations and other hormonal parameters in obese and non-obese women with polycystic ovary syndrome
- P1-IS-3 Predictive and prognostic role of activated Akt/mTOR in cervical cancer treated with cisplatin-based neoadjuvant chemotherapy
- P-IS-32 Inhibition of the mammalian target of rapamycin--mTOR--by rapamycin increases the chemo-sensitivity of CaSki cell to paclitaxel(Oncology 8,Group 95,International Session)
- The Stimulatory Effect of Activin on PRL Production in GH3 Cells in a Serum-Free Culture
- ARRHYTHMIA MONITOR : PROCEEDINGS OF THE 37TH ANNUAL MEETING OF THE JAPANESE CIRCULATION SOCIETY
- Changes in FSH, LH and Prolactin Secretion During Estrous Cycle in Rats
- The Presence of LH Components Having Different Ratios of Bioactivity to Immunoreactivity in the Rat Pituitary Glands
- Immunological and Biological Characteristics of a New TRF Analogue, L-Pyroglutamyl-L-Histidyl-L-Pipecolic Acid Amide
- Measurement of Human FSH by Radioreceptor Assay
- Species Specificity of Radioreceptor Assay and Radioimmunoassay for Rat FSH
- Effect of Gonadectomy and Steroid Treatment on the Receptor-Binding Activity and Immunoreactivity of Serum and Pituitary FSH in the Adult Rats of Both Sexes
- Measurement of Rat Serum FSH by Radioreceptor Assay and Comparison with Radioimmunoassay
- On the Presence of FSH-β Subunit in the Rat Anterior Pituitary and Serum
- Effect of Prostaglandin F2α upon Ovulation and LH, FSH and Prolactin Secretion in Chlorpromazine Blocked Rats
- Seasonal Variation in Pituitary Gonadotropin in the Adult Male Newt, Cynops Pyrrhogaster Pyrrhogaster, Revealed by Isoelectric Focusing Technique and Radioreceptor Assay
- Effects of X-Ray Irradiation on the Subsequent Gonadotropin Secretion in Normal and Neonatally Estrogenized Female Rats
- Heterogeneity of Rat Luteinizing Hormone Revealed by Radioimmunoassay and Electrofocusing Studies
- A Sensitive Radioreceptor Assay for Follicle Stimulating Hormone with PMS-primed Immature Rat Ovary
- A New More Potent Analogue of LH-RH : [Des-Gly-NH210, Pro-ethylamide9]-LH-RH
- Circadian Rhythms in Serum LH, FSH and Prolactin Levels in Adult Male Rats
- Different Profiles of Isoelectric Avian Luteinizing Hormone Components in Biological Activity and Immunoreactivity
- Radioimmunoassay. Problems of Measurement of Gonadotropins in Blood Samples
- Acute and Subacute Effect of LH-RH upon LH-and FSH-Gonadotrophs in Castrated Female Rats with Short-Term Estrogen-Progesterone Pretreatment
- Chronic Effect of TRH and LRH upon a Series of Basophils along with the Serum and Pituitary TSH, LH and FSH Concentration
- Short-and Auto-Feedback Mechanism of LH
- Effect of AA560 (A Nonsteroidal Antiandrogen) Implantation In the Hypothalamus on Gonadotropin Secretion in Male Rats
- Serum Prolactin Levels during Ovulatory Menstrual Cycle and Menstrual Disorders in Women
- Role of Prostaglandin F2α in Ovulation
- Action of a novel nonsteroidal antiandrogen, AA560.
- Approach to the Treatment of Ascites Associated with Ovarian Hyperstimulation Syndrome.
- Clinical Factors in Successful Transfer of Cryopreserved Thawed Embryos.
- Epididymal and Testicular Sperm for Intracytoplasmic Sperm Injection in the Treatment of Azoospermia.
- A Case of Empty Follicle Syndrome.