The Presence of LH Components Having Different Ratios of Bioactivity to Immunoreactivity in the Rat Pituitary Glands
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概要
- 論文の詳細を見る
The isoelectric components of LH have been isolated from the male rat pituitary glands by preparative isoelectric focusing. The biological activities of thesecomponents were measured <I>in vitro</I> from testosterone production in rat Leydig cells at equal doses and expressed as the equivalent of NIH-LH-Sl immunoreactivity. There were significant differences in the bioactivities among the components. The bioactivities of the components decreased with decreasing pI; components E (pI=9.6) and F (pI=9.8) showed about 5-to 6-fold higher activities than component A(PI=7.9). When the rat pituitary extracts were measured for LH by radioimmunoassay, and then measured for the ratio of biological activity to immunoreactivity (B: I), theorder was as follows; intact female rats>intact male rats>orchidectomized rats. This phenomenon could be explained by the differences in the relative amounts of the LH components which changed according to the physiological states. These observations indicate that the LH components with different pis and B: I ratio are related to the different estimation of total LH in the rat pituitary glands by bioassay and radioimmunoassay.
- 社団法人 日本内分泌学会の論文
著者
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Hattori M
Department Of Animal And Marine Bioresource Sciences Graduate School Of Agriculture Kyushu Universit
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Hattori Masa-aki
Hormone Assay Center Institute Of Endocrinology Gunma University
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Hattori M
Kyushu Univ. Fukuoka Jpn
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Hattori M
Hormone Assay Center Institute Of Endocrinology Gunma University
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Wakabayashi Katsumi
Hormone Assay Center Institute of Endocrinology Gunma University
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Sakamoto Kenji
Hormone Assay Center Institute of Endocrinology Gunma University
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Wakabayashi K
Gunma Univ. Gunma Jpn
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Wakabayashi Katsumi
Hormone Assay Center Institute Of Endocrinology Gumma Univ.
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SAKAMOTO KENJI
Hormone Assay Center, Institute of Endocrinology, Gunma University
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