Temporal and Spatial Differential Expression of Chicken Germline-specific Proteins cDAZL, CDH and CVH During Gametogenesis
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概要
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The Deleted in Azoospermia-Like (DAZL) protein coded by Dazl gene is a germline-specific RNA-binding protein essential for gametogenesis in vertebrates, and the chicken Dazl gene has also been identified in primordial germ cells (PGCs). However, the temporal and spatial expression of chicken DAZL (cDAZL) and its molecular role in germ cell development remain enigmatic. Here, we investigated the subcellular distribution and expression of cDAZL at the various stages by using a polyclonal antibody raised against its C-terminal region and compared them with those of additional germline-specific proteins chicken vasa homologue (CVH) and chicken dead end homologue (CDH). Western blot analysis for cDAZL revealed a single band in the embryonic gonads and premature chicken testis, whereas no band was detected in the premature chicken ovary. Fluorescent immunohistochemistry revealed that cDAZL was present in the nucleus and cytoplasm of circulating PGCs. Cells positive for cDAZL and CVH coexisted in the embryonic gonads and premature chicken testis, in which they were distributed near the basement membrane of seminiferous tubules. Of interest, cDAZL was not found in the premature chicken ovary, whereas CVH and CDH were present in germ cells. Collectively, three germline-specific proteins are expressed in chicken germ cells, but their patterns of expression are temporally and spatially distinct.
- 2010-06-01
著者
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TANAKA Koji
Laboratory Animal Science & Toxicology Laboratories, Sankyo Co., Ltd.
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KITO Gakushi
Laboratory of Reproductive Physiology and Biotechnology, Department of Animal and Marine Bioresource
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ARAMAKI Shinya
Laboratory of Reproductive Physiology and Biotechnology, Department of Animal and Marine Bioresource
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SOH Tomoki
Laboratory of Reproductive Physiology and Biotechnology, Department of Animal and Marine Bioresource
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YAMAUCHI Nobuhiko
Laboratory of Reproductive Physiology and Biotechnology, Department of Animal and Marine Bioresource
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HATTORI Masa-aki
Laboratory of Reproductive Physiology and Biotechnology, Department of Animal and Marine Bioresource
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Soh T
Department Of Animal And Marine Bioresource Sciences Graduate School Of Agriculture Kyushu Universit
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Soh Tomoki
Kyushu Univ. Fukuoka
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Hattori M
Department Of Animal And Marine Bioresource Sciences Graduate School Of Agriculture Kyushu Universit
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Soh Tomoki
九州大学 農学研究院動物資源科学
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Soh Tomoki
Laboratory Of Reproductive Physiology Division Of Animal Science Department Of Animal And Marine Bio
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Aramaki Shinya
九州大学 農学研究院動物資源科学
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Yamauchi N
Department Of Animal And Marine Bioresource Sciences Graduate School Of Agriculture Kyushu Universit
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Yamauchi Nobuhiko
九州大学 農学研究院動物資源科学
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Yamauchi Nobuhiko
Laboratory Of Reproductive Biology And Technology Department Of Developmental Biology National Insti
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Hattori Masa-aki
Laboratory Of Reproductive Physiology And Biotechnology Department Of Animal And Marine Bioresource
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Soh Tomoki
Laboratory Of Reproductive Physiology And Biotechnology Department Of Animal And Marine Bioresource
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Aramaki Shinya
Laboratory Of Reproductive Physiology And Biotechnology Department Of Animal And Marine Bioresource
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Kito Gakushi
Laboratory Of Reproductive Physiology And Biotechnology Department Of Animal And Marine Bioresource
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Hattori M
Kyushu Univ. Fukuoka Jpn
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Hattori M
Hormone Assay Center Institute Of Endocrinology Gunma University
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Yamauchi N
Tohoku Univ. Sendai Jpn
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Yamauchi Nobuhiko
Laboratory Of Animal Reproduction Department Of Animal Science Faculty Of Agriculture Tohoku Univers
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Soh T
Laboratory Of Reproductive Physiology And Biotechnology Department Of Animal And Marine Bioresource
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Tanaka Koji
Laboratory Of Reproductive Physiology And Biotechnology Department Of Animal And Marine Bioresource
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