Production and Characterization of an Antiserum against Recombinant Porcine Follicle Stimulating Hormone
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概要
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This study aimed to develop an antiserum specific to follicle stimulating hormone (FSH), for a wide range of species, free from cross-reactivity against luteinizing hormone. An antiserum against recombinant porcine FSH (rec-pFSH) expressed in insect cells was raised in a rabbit and its specificity was characterized by time-resolved fluoroimmunoassay (TR-FIA) and immunohistochemistry. The titer of the anti-rec-pFSH antiserum was about 1:5,000 for use in TR-FIA. The inhibition curve of TR-FIA with rec-pFSH paralleled that of pituitary-derived FSH, indicating that the antigenicities of the two FSH preparations do not differ significantly. The anti-rec-pFSH antiserum showed low reactivities with FSHs prepared from sheep and rat, suggesting it was species- and hormone-specific, contrary to our intention. The TR-FIA showed that this antiserum did not react with pituitary extracts from mammals other than the pig. However, immunohistochemistry demonstrated that this antiserum reacted with the specific cells not only of the porcine anterior pituitary but also of the cow, goat, and horse. The antiserum also immunostained pituitary cells of the cow, goat and horse. Therefore, this anti-rec-pFSH antiserum will be of limited use in studies involving competitive immunoreactions due to its species-specific reactivity, but it should be useful for non-competitive techniques, such as immunohistochemistry.
著者
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KATO Takako
Biosignal Research Center, Institute for Molecular and Cellular Regulation, Gunma University
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TOMIZAWA Kyoko
Biosignal Research Center, Institute for Molecular and Cellular Regulation, Gunma University
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Hattori Masa-aki
Laboratory Of Reproductive Physiology And Biotechnology Department Of Animal And Marine Bioresource
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Ihara Takeshi
Nippon Institute For Biological Science
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SATO Ichiro
Nippon Institute for Biological Science
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UEDA Susumu
Nippon Institute for Biological Science
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Inoue Kinji
Department Of Cell Biology Faculty Of Science Saitama University
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Tomizawa Kyoko
Biosignal Research Center Institute For Molecular And Cellular Regulation Gunma University
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Kato Yukio
Biosignal Research Center Institute For Molecular And Cellular Regulation Gunma University
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SAKAI Takafumi
Department of Applied Microbial Technology, The Kumamoto Institute of Technology
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Sakai Takafumi
Department of Regulation Biology, Faculty of Science, Saitama University
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WATANABE Miyuki
Department of Orthodontics, School of Dentistry, Showa University
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MORI Junichi
Theriogenology, School of Veterinary Medicine and Animal Sciences, Kitasato University
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OGAWA Satoshi
Biosignal Research Center, Institute for Molecular and Cellular Regulation, Gunma University
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KIKUCHI Motohiro
Theriogenology, School of Veterinary Medicine and Animal Sciences, Kitasato University
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WATANABE Miyuki
Department of Regulation Biology, Faculty of Science, Saitama University
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