Production of Interspecific Germline Chimera between Quail and Chicken Utilizing the Cells from Central Disk of Blastoderm and Germinal Crescent Region

概要

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Production of interspecific germline chimera between quail and chicken was carried out using the cells from the central disk (CD) of the area pellucida in quali blastoderm (stage X) and the germinal crescent region (GCR) of quali embryo (stage7-8). The cells of CD and GCR were dispersed and injected into the subgerminal cavity of chicken blastoderm (stage X). Injected aggs were incubated for 7 days or to hatching. To detect quali genomic DNA, the polymerate chain reaction was used. In day-7 embryos, quali DNA was detected in 4 gonads and 6 brains from 55 survived embryos received quali CD cells, and 9 gonads and 8 brains from 68 survived embryos received quali GCR cells. Quali DNA was also detected from eggs or semen of adult female or male chicken hatched from eggs received quali CD or GCR cells. CD and GCR cells as the donors showed the possibility to produce the interspecific germiline chimera but the necessary improevment and the immunoreactive problem were suggested.
九州大学の論文