Functional regions of organic cation/carnitine transporter OCTN2 (SLC22A5): Roles in carintine recognistion

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概要

• 論文の詳細を見る

• 出版者許諾要件により全文は非公開.The organic cation/carnitine transporter OCTN2 transports carnitine in a sodium-dependent manner, whereas it transports organic cations sodium-independently. To elucidate the functional domain in OCTN2, we constructed chimeric proteins of human OCTN2 (hOCTN2) and mouse OCTN3 (mOCTN3) and introduced mutations at several amino acids conserved among human, rat and mouse OCTN2. We found that transmembrane domains (TMD) 1-7 are responsible for organic cation transport and for sodium dependence in carnitine transport. Within TMD1-7, Q180 and Q207 of hOCTN2 are the critical amino acids for the sodium dependence, and double mutation of Q180 and Q207 resulted in minimal change in transport activity when sodium was removed from the uptake medium. We propose that sodium-dependent affinity for carnitine is dependent on sodium recognition by these critical amino acids in hOCTN2, whereas carnitine transport by OCTN2 requires functional linkage between TMD1-7 and TMD11.

• The Japanese Society for the Study of Xenobiotics = 日本薬物動態学会の論文
• 2004-06-30

著者

• Tsuji Akira

  Faculty Of Pharmaceutical Sciences Kanazawa University

• Tsuji Akira

  金沢大学 自然科学研究科創剤科学

• Tsuji A

  金沢大学 自然科学研究科創剤科学

• Kubo Yoshiyuki

  金沢大学 自然科学研究科創剤科学

• Sai Yoshimichi

  Division Of Pharmaceutical Sciences Graduate School Of Natural Science & Technology

• Sai Yoshimichi

  Faculty Of Pharmaceutical Sciences Kanazawa University

• Tamai I

  Tokyo Univ. Sci. Tokyo Jpn

• Tamai Ikumi

  Meiji Seika Kaisha Ltd. Pharmaceutical Research Center:department Of Surgery Kawasaki City Ida Hospi

• Tamai Ikumi

  Faculty Of Pharmaceutical Sciences Tokyo University Of Science

• Tamai Ikumi

  Department Of Pharmaceutics Faculty Of Pharmaceutical Sciences Kanazawa University

• Tamai Ikumi

  Faculty Of Pharmaceutical Sciences And Hospital Pharmacy Kanazawa University

• ISHIGURO Masaji

  Suntory Institute for Bioorganic Research

• KATO Yukio

  Faculty of Pharmaceutical Sciences, Kanazawa University

• INANO Akihiro

  Faculty of Pharmaceutical Sciences, Kanazawa University

• Kato Yukio

  金沢大学 自然科学研究科創剤科学

• Kato Yukio

  Faculty Of Agriculture Meiji University

• Kubo Yoshiyuki

  Faculty Of Pharmacy Institute Of Medical Pharmaceutical And Health Sciences Graduate School Of Medic

• Sai Y

  Division Of Pharmaceutical Sciences Graduate School Of Natural Science & Technology

• Inano Akihiro

  Faculty Of Pharmaceutical Sciences Kanazawa University

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