Differential Expression of Ezrin and CLP36 in the Two Layers of Syncytiotrophoblast in Rats
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概要
- 論文の詳細を見る
The syncytiotrophoblast, which regulates maternal–fetal transfer of drugs, consists of a single layer in humans, but two layers, i.e., SynI and SynII, in rodents. Polar distribution of transporters in the apical and basal plasma membranes of syncytiotrophoblast is important for placental function in terms of vectorial transport of substrates, but the mechanisms that control protein distribution in the syncytiotrophoblast remain unclear. We have previously established rat syncytiotrophoblast cell lines, TR-TBT 18d-1 and TR-TBT 18d-2, which retain characteristics of SynI and SynII, respectively. In this study, we aimed to characterize the gene expression profiles in the two layers by using these cell lines. DNA microarray analysis indicated that more than 25 mRNAs, including cytoskeleton binding proteins, ezrin and CLP36, are differentially expressed between TR-TBT 18d-1 and TR-TBT 18d-2. Quantitative real time-polymerase chain reaction (PCR) analysis indicated that mRNA expression of ezrin, CLP36, CCN1, and CCN2 is higher in TR-TBT 18d-1 and mRNA expression of elf-1a, hsc70 and flot2 is higher in TR-TBT 18d-2, compared with their counterparts. Immunohistochemical analysis indicated that ezrin is expressed in rat placental villi in vivo, and is located on the apical membranes of TR-TBT 18d-1, while CLP36 is located in the apical and basal sides of TR-TBT 18d-1. The expression of ezrin was highest at gestational days 14 and 18 and was highest among the ezrin/radixin/moesin (ERM) family members. These results may help to clarify the mechanisms controlling polarization of the syncytiotrophoblast and the significance of the double epithelial layers in rat and mouse.
著者
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Wakayama Tomohiko
Department Of Histology And Embryology Graduate School Of Medical Science Kanazawa University
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SAI Yoshimichi
Division of Pharmaceutical Sciences, Graduate School of Natural Science & Technology
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Higuchi Kei
Division of Pharmaceutics, Faculty of Pharmacy, Keio University
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Iizasa Hisashi
Division of Pharmaceutics, Faculty of Pharmacy, Keio University
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Horieya Satomi
Division of Pharmaceutics, Faculty of Pharmacy, Keio University
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Lee Kyeong-Eun
Division of Pharmaceutics, Faculty of Pharmacy, Keio University
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Wada Masami
Division of Pharmaceutics, Faculty of Pharmacy, Keio University
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Deguchi Masayuki
Division of Pharmaceutics, Faculty of Pharmacy, Keio University
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Nishimura Tomohiro
Division of Pharmaceutics, Faculty of Pharmacy, Keio University
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Tamura Atsushi
Laboratory of Biological Science, Graduate School of Frontier Biosciences, Osaka University
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Tsukita Sachiko
Laboratory of Biological Science, Graduate School of Frontier Biosciences, Osaka University
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Kose Noriko
Division of Pharmaceutics, Faculty of Pharmacy, Keio University
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Kang Young-Sook
College of Pharmacy, Sookmyung Womens University
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Nakashima Emi
Division of Pharmaceutics, Faculty of Pharmacy, Keio University
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Wakayama Tomohiko
Department Of Anatomy And Reproductive Cell Biotogy Miyazaki Medicat Couege
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Sai Yoshimichi
Division Of Pharmaceutical Sciences Graduate School Of Natural Science & Technology
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Kose Noriko
Department Of Pharmaceutics Kyoritsu University Of Pharmacy
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Nakashima E
Dep. Of Pharmaceutics Kyoritsu Univ. Of Pharmacy
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Nakashima Emi
Faculty Of Pharmaceutical Sciences Kanazawa University
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Nakashima Emi
Department Of Pediatrics:hospital Pharmacy:and Faculty Of Pharmaceutical Sciences University Of Kana
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Iizasa H
Kyoritsu Univ. Pharmacy Tokyo Jpn
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Sai Yoshimichi
Department Of Pharmaceutics Kyoritsu University Of Pharmacy
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中島 恵美
共立薬科大学 薬剤学講座
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Nakashima Emi
Faculty Of Pharmaceutical Sciences And Hospital Pharmacy Kanazawa University
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Kang Young-sook
College Of Pharmacy And Research Institute Of Pharmaceutical Sciences Sookmyung Women's Univers
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Suwa Toshio
Department Of Drug Development Science & Clinical Evaluation Kyoritsu University Of Pharmacy
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中島 恵美
Department Of Pharmaceutics Kyoritsu University Of Pharmacy
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Wakayama Tomohiko
Department of Histology and Embryology, Graduate School of Medical Sciences, Kanazawa University
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Kang Young-Sook
College of Pharmacy, Sookmyung Women's University
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