Conditionally Immortalized Syncytiotrophoblast Cell Lines as New Tools for Study of the Blood-Placenta Barrier
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概要
- 論文の詳細を見る
Syncytiotrophoblasts play an essential role in restriction of drug delivery through the blood-placenta barrier (BPB). Conditionally immortalized Syncytiotrophoblast cell lines, TR-TBTs, were established at gestational day 18 from pregnant transgenic rats (Tg-rats) harboring the temperature-sensitive SV 40 (ts SV40) large T-antigen. TR-TBTs exhibit temperature-sensitive cell growth due to the expression of SV 40 large T-antigen, and thus the cell growth can be regulated by changing the culture temperature. TR-TBTs exhibit typical properties of Syncytiotrophoblast cells, such as syncytium-like morphology, the expression of cytokeratins and hormones, and polarized expression of glucose transporter 1 (GLUT1) and GLUT3. TR-TBTs express in vivo influx and efflux trans- porters, such as taurine transporter (TauT), betaine/GABA transporter (BGT-1), amino acid transporter 2 (ATA2), organic anion transporting polypeptide 2 (oatpZ), organic cation/carnitine transporter (OCTN2), P-gly- coprotein (P-gp), and breast cancer resistance protein (BCRP/ABCG2). Moreover, TR-TBTs exhibit taurine, GABA, and DHEA-S uptake activity via TauT, BGT-1, and oatp2, respectively. Therefore, TR-TBTs can be used for the analysis of these functions and would be a good in vitro models for investigating carrier-mediated trans- port functions at the BPB.
- 公益社団法人日本薬学会の論文
- 2004-06-01
著者
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MAEDA Tomomi
Department of Internal Medicine, Teikyo University School of Medicine
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Maeda T
Department Of Pharmaceutics Kyoritsu University Of Pharmacy
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Nakashima Emi
Department Of Pharmaceutics Kyoritsu University Of Pharmacy
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Nakashima Emi
Department Of Pediatrics:hospital Pharmacy:and Faculty Of Pharmaceutical Sciences University Of Kana
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Iizasa Hisashi
Department Of Pharmaceutics Kyoritsu University Of Pharmacy
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HIROSE Yoko
Department of Molecular Medicinal Sciences, Graduate School of Biomedical Sciences, Nagasaki Univers
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Hirose Y
Department Of Molecular Medicinal Sciences Graduate School Of Biomedical Sciences Nagasaki Universit
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Maeda Tomomi
Department Of Internal Medicine Teikyo University School Of Medicine
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Hwang In-won
Department Of Pharmaceutics Kyoritsu University Of Pharmacy
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KITANO Tomohide
Department of Pharmaceutics, Kyoritsu University of Pharmacy
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MORITA Tomonari
Department of Pharmaceutics, Kyoritsu University of Pharmacy
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Morita Tomonari
Department Of Pharmaceutics Kyoritsu University Of Pharmacy
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Kitano Tomohide
Department Of Pharmaceutics Kyoritsu University Of Pharmacy
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