Expression of Integrin Subunits Depend on Bovine Endometrial Stromal Cells Cultured In Vitro
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概要
- 論文の詳細を見る
Expression patterns of four integrin α subunits (α2, α3, α5 and αV) and two integrin β subunits (β1 and β3) were examined in bovine endometrial stromal cells (BES) by reverse transcription-polymerase chain reaction and indirect immunofluorescence analysis. All six integrin subunit genes were expressed in the bovine endometrium and in in vitro cultured BES, except α2 subunit gene, which was not expressed with increased generation and longer culture period. The α5 and αV integrin subunit proteins were detected in cultured BES. The α2, α3 and β1 subunit proteins were undetectable in BES derived from pregnant endometrium ; however, these subunits are expressed in vivo. These results demonstrate that the expression patterns of integrin subunits are different between endometrium and cultured cells in terms of both mRNA and protein levels. It is indicating that culture conditions influence the expression of integrins in BES.
- 日本結合組織学会の論文
- 2003-03-25
著者
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HASHIZUME Kazuyoshi
Laboratory of Reproductive Endocrinology, National Institute Animal Industry
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Yamauchi N
Laboratory Of Reproductive Biology And Technology Depar
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Yamauchi Nobuhiko
農業生物資源研究所
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Yamauchi Nobuhiko
Laboratory Of Reproductive Biology And Technology Department Of Developmental Biology National Insti
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橋爪 一善
農林水産省畜産試験場
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橋爪 一善
農業生物資源研究所 発生分化研究グループ 生殖再生研究チーム
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Herath C
Laboratory Of Reproductive Biology And Technology Depar
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Hashizume K
National Inst. Animal Ind. Ibaraki
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Hashizume Kazuyoshi
Laboratory Of Animal Reproduction Faculty Of Agriculture Tohoku University
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KIZAKI Keiichiro
Laboratory of Reproductive Biology and Technology, Depar
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YAMADA Osamu
Laboratory of Reproductive Biology and Technology, Depar
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TAKAHASHI Toru
Laboratory of Reproductive Biology and Technology, Depar
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HERATH Chandana
Laboratory of Reproductive Biology and Technology, Depar
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Yamauchi Nobuhiko
Laboratory Of Animal Reproduction Department Of Animal Science Faculty Of Agriculture Tohoku Univers
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Hashizume K
Laboratory Of Reproductive Biology And Technology Depar
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Yamato Osamu
Kagoshima Univ. Kagoshima
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Yamada O
Laboratory Of Clinical Pathology Department Of Veterinary Clinical Sciences Faculty Of Agriculture K
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Hashizume Kazuyoshi
Reproductive Biology And Technology Laboratory Developmental Biology Department National Institute O
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Kizaki Keiichiro
Laboratory Of Reproductive Biology And Technology Depar
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Takahashi T
Laboratory Of Reproductive Biology And Technology Depar
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