Hormonal Differences in Peripheral Blood and Gene Profiling in the Liver and Lymphocytes in Japanese Black Cattle with Growth Retardation

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概要

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• Japanese Black cattle occasionally demonstrate growth retardation despite sufficient nutrient intake. To clarify hormonal and transcriptional characteristics, we investigated differences in blood components, including hormones, and differences in exhaustive gene expressions in the liver and peripheral lymphocytes of six cattle with growth retardation (GR cattle) and eight control cattle of the same age and pedigree with normal growth. Hematocrit values and concentrations of hemoglobin, serum albumin, total cholesterol, insulin-like growth factor 1 (IGF-1), thyroxine, and insulin in GR cattle were significantly lower than those in controls. GR cattle also excreted higher levels of GH. We used three GR and three control cattle in microarray analysis in the liver and found that 279 gene expressions were significantly different. However, GH-IGF-1 axis related gene expressions, such as the GH receptor and IGF-1, were not significantly different from those of controls. Immune related gene expressions were significantly lower. To clarify these gene expression levels, peripheral lymphocytes were used for real-time RT-PCR. The expression rates of genes that were significantly lower in the liver, such as chemokine ligand 8, interferon gamma receptor 1, and immunoglobulin light chain VJ region were also significantly lower in three GR cattle than those in three control cattle. These results suggest that the cause of growth retardation in the present study was due to other factors, not abnormal gene expressions of GH-IGF-1 axis related factors in the liver, and that GR cattle were susceptible to infectious disease.

著者

• Hashizume Kazuyoshi

  Laboratory Of Animal Reproduction Faculty Of Agriculture Tohoku University

• Yonezawa Tomohiro

  Laboratory Of Veterinary Physiology Kitasato University

• Kizaki Keiichiro

  Laboratory Of Reproductive Biology And Technology Depar

• Kurose Yohei

  Laboratory Of Animal Nutrition Faculty Of Animal Science Kitasato University

• Hirano Takashi

  Shirakawa Institute Of Animal Genetics Japan Livestock Technology Association

• ISHIDA Sakura

  Laboratory of Large Animal Surgery, School of Veterinary Medicine, Kitasato University, 23–35–1 Higashi, Towada, Aomori 034–8628, Japan

• EIRAI Sayoko

  Nara Prefectural Livestock Hygiene Service Center, 152–1 Minamijyuso, Gose, Nara 639–2204, Japan

• TOMIOKA Michiko

  Laboratory of Large Animal Surgery, School of Veterinary Medicine, Kitasato University, 23–35–1 Higashi, Towada, Aomori 034–8628, Japan

• WATANABE Daisaku

  Laboratory of Large Animal Surgery, School of Veterinary Medicine, Kitasato University, 23–35–1 Higashi, Towada, Aomori 034–8628, Japan

• HIRANO Takashi

  Shirakawa Institute of Animal Genetics, Odakura, Nishigo, Fukushima 961–8061, Japan

• HASHIZUME Kazuyoshi

  Laboratory of Veterinary Physiology, Iwate University, 3–18–8 Ueda, Morioka, Iwate 020–8550, Japan

• YONEZAWA Tomohiro

  Laboratory of Veterinary Physiology, School of Veterinary Medicine, Kitasato University, 23–35–1 Higashi, Towada, Aomori 034–8628, Japan

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