時間分解蛍光免疫測定によるニホンジカ血漿プロジェステロン濃度の直接測定法

概要

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A direct time-resolved fluorescent immunoassay (TR-FIA) system was developed and evaluated for measuring progesterone (P) concentrations in deer plasma. The minimum detectable level of this assay system was 0.25 ng/ml and ED 50 value of standard curve was 2.4 ng/ml. Intra- and inter-assay coefficients of variation were 8.8% and 15.7%, respectively. The direct TR-FIA revealed that the plasma P concentrations in sika deer expressed a diurnal pattern depending on their reproductive status. Results in the present study indicate that the TR-FIA system, that is specifically designed for humans if successfully utilized with slight modification, can be utilized for direct assaying of P in deer plasma. The plasma P profile in the sika deer appears to be consistent to that of other temperate deer expressing seasonal breeding characteristics.
日本繁殖生物学会の論文