Cloning and Heterologous Expression of the Antibiotic Peptide (ABP) Genes from Rhizopus oligosporus NBRC 8631

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概要

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• We carried out protein sequencing of purified Antibiotic Peptide (ABP), and cloned two genes encoding this peptide as abp1 and abp2, from Rhizopus oligosporus NBRC 8631. Both genes contain an almost identical 231-bp segment, with only 3 nucleotide substitutions, encoding a 77 amino acid peptide. The abp gene product comprises a 28 amino acid signal sequence and a 49 amino acid mature peptide. Northern blot analysis showed that at least one of the abp genes is transcribed in R. oligosporus NBRC 8631. A truncated form of abp1 encoding only the mature peptide was fused with the α-factor signal peptide and engineered for expression in Pichia pastoris SMD1168H. Culture broth of the recombinant Pichia displayed ABP activity against Bacillus subtilis NBRC 3335 after induction of heterologous gene expression. This result indicates that mature ABP formed the active structure without the aid of other factors from R. oligosporus, and was secreted.

• 社団法人 日本農芸化学会の論文
• 2005-03-23

著者

• Yamada Osamu

  National Cardiovascular Center

• KOSEKI Takuya

  National Research Institute of Brewing

• AKITA Osamu

  National Research Institute of Brewing (NRIB)

• Sakamoto K

  Fermentation Research Laboratories Fujisawa Pharmaceutical Co. Ltd.

• Sakamoto Kazutoshi

  National Research Institute Of Brewing

• Akita O

  Fac. Of Human Life Sciences Dep. Of Food And Health Sciences Jissen Women's Univ.

• Akita Osamu

  National Research Institute Of Brewing

• Fujita Akiko

  National Research Institute Of Brewing

• Yamato Osamu

  Kagoshima Univ. Kagoshima

• Yamada O

  Laboratory Of Clinical Pathology Department Of Veterinary Clinical Sciences Faculty Of Agriculture K

• TOMINAGA Mihoko

  National Research Institute of Brewing

• NAKAYAMA Tasuku

  Faculty of Engineering, Hiroshima University

• Nakayama Tasuku

  Faculty Of Engineering Hiroshima University

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