Cloning and Enhanced Expression of the Cytochrome P450nor Gene (nicA ; CYP55A5) Encoding Nitric Oxide Reductase from Aspergillus oryzae
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概要
- 論文の詳細を見る
We cloned and characterized the gene and cDNA of Aspergillus oryzae cytochrome P450nor (Anor). The Anor gene (nicA; CYP55A5) has a different gene structure from other P450nor genes in that it has an extra intron. There were not only two kinds of mRNA but also two sets of TATA-box and CCAAT-box, and it appears that this gene has two expression patterns, like CYP55A1 of Fusarium oxysporum. A reporter analysis using the uidA gene indicated that gene expression of CYP55A5 was induced under anaerobic conditions, like CYP55A1. When the CYP55A5 gene was overexpressed in A. oryzae, a large amount of active Anor were accumulated as intracellular protein. Anor employed both NADH and NADPH as electron donors for reducing nitric oxide to nitrous oxide. Anor measured the amount of NO generated from 3-(2-Hydroxy-1-(1-methylethyl)-2-nitrosohydrazino)-1-propanamine (NOC5) with a spectrophotometer. The sensitivity was 10 nmol/ml.
- 社団法人 日本農芸化学会の論文
- 2004-10-23
著者
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Hata Yoji
Research Institute, Gekkeikan Sake Co. Ltd.
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SHOUN Hirofumi
Department of Biotechnology, The University of Tokyo
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Takaya Naoki
Institute Of Applied Biochemistry University Of Tsukuba
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Hata Yoji
Res. Inst. Gekkeikan Sake Co. Ltd. 101 Shimotoba-koyanagi-cho Fushimi-ku Kyoto 612-8385 Jpn
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Abe Yasuhisa
Res. Inst. Gekkeikan Sake Co. Ltd.
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Abe Yasuhisa
Research Institute For Fundamental Physics Kyoto University : Centre De Recherches Nucleaires
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Abe Yasuhisa
Research Institute For Fundamental Physics Kyoto University
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KAYA Masahiko
Research Institute, Gekkeikan Sake Co., Ltd.
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MATSUMURA Kengo
Research Institute, Gekkeikan Sake Co., Ltd.
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HIGASHIDA Katsuya
Research Institute, Gekkeikan Sake Co., Ltd.
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KAWATO Akitsugu
Research Institute, Gekkeikan Sake Co., Ltd.
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AKITA Osamu
National Research Institute of Brewing (NRIB)
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Shoun Hirofumi
Department Of Biotechnology Graduate School Of Agricultural And Life Sciences University Of Tokyo
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Shoun Hirofumi
Department Of Agricultural Chemistry The University Of Tokyo:(present Office)institute Of Applied Bi
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Hata Yoji
Research Institite Of Gekkeikan Gekkeikan Sake Co. Ltd.
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Abe Yasushisa
Research Institite Of Gekkeikan Gekkeikan Sake Co. Ltd.
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Akita O
Fac. Of Human Life Sciences Dep. Of Food And Health Sciences Jissen Women's Univ.
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Akita Osamu
National Research Institute Of Brewing
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Kaya Masahiko
Research Institute Gekkeikan Sake Co. Ltd.
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Kawato A
Research Institute Gekkeikan Sake Co. Ltd.
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Kawato Akitsugu
Research Institute Gekkeikan Sake Co. Ltd.
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Abe Yasuhisa
Research Institute Gekkeikan Sake Co. Ltd.
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Matsumura Kengo
Research Institute Gekkeikan Sake Co Ltd.
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Higashida Katsuya
Research Institute Gekkeikan Sake Co. Ltd.
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Abe Yasuhisa
Research Center For Nuclear Physics Osaka University
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