Molecular Cloning and Overexpression of fleA Gene Encoding a Fucose-specific Lectin of Aspergillus oryzae(Biochemistry & Molecular Biology)
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概要
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A protein from the cell lysate of Aspergillus oryzae was purified by column chromatography immobilized with a ferrichrysin (Fec), which is one of the siderophores of A. oryzae. It is produced only in an iron-deficient culture and its molecular weight is estimated as 35,000 by SDS-PAGE. Two internal amino acid sequences of the protein obtained by lysylendopeptidase digestion were analyzed. Molecular cloning shows that it encodes 310 putative amino acid residues separated by 4 introns and is designated as fleA. It shows approximately 26% similarity with the gene encoding a fucose-specific lectin of Aleuria aurantia (AAL). The gene was overexpressed under control of the melO promoter in a submerged culture of A. oryzae. The fleA gene product showed hemagglutination activity against rabbit erythrocytes. A hemagglutination inhibition assay of monosaccharides showed that this lectin specifically binds to L-fucose and weakly reacts with mannose and N-acetyl-neuraminic acid.
- 社団法人日本農芸化学会の論文
- 2002-05-23
著者
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Hata Yoji
Res. Inst. Gekkeikan Sake Co. Ltd. 101 Shimotoba-koyanagi-cho Fushimi-ku Kyoto 612-8385 Jpn
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Ishida H
Research Institute Gekkeikan Sake Co. Ltd.
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Ishida Hiroki
Research Institute Gekkeikan Sake Co. Ltd.
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Abe Yasuhisa
Res. Inst. Gekkeikan Sake Co. Ltd.
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Abe Yasuhisa
Research Institute For Fundamental Physics Kyoto University
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Hata Yoji
Research Institite Of Gekkeikan Gekkeikan Sake Co. Ltd.
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IMAYASU Satoshi
Research Institite of Gekkeikan, Gekkeikan Sake Co., Ltd.
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Abe Yasushisa
Research Institite Of Gekkeikan Gekkeikan Sake Co. Ltd.
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SUGINAMI Koji
Research Institute, Gekkeikan Sake Co. Ltd.
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Kawato A
Research Institute Gekkeikan Sake Co. Ltd.
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Kawato Akitsugu
Research Institute Gekkeikan Sake Co. Ltd.
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Imayasu Satoshi
Research Institute Gekkeikan Sake Co. Ltd.
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Suginami K
Research Institute Gekkeikan Sake Co. Ltd.
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Suginami Koji
Research Institute Gekkeikan Sake Co. Ltd.
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Abe Yasuhisa
Research Institute Gekkeikan Sake Co. Ltd.
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MORITANI Toshiyasu
Research Institute, Gekkeikan Sake Co. Ltd.
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Moritani Toshiyasu
Research Institute Gekkeikan Sake Co. Ltd.
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Abe Yasuhisa
Research Center For Nuclear Physics Osaka University
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