Adsorption of Glucoamylase in Unpasteurized Sake on an Affinity Column of Immobilized Glucoamylase Inhibitors
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概要
- 論文の詳細を見る
Affinity chromatography using an immobilized glucoamylase inhibitors (GAIs) column promises glucoamylase(GA)-free unpasteurized sake by means of complete adsorption of the GA in unpasteurized sake on the comumn. In consequence of the adsorption taking place at a maximum flow rate (SV=90h^<-1> at 4℃, even in the presence of 2% (w/v) glucose and 20% (v/v) ethanol, any change in the quality of the unpasteurized sake could be maximally suppressed during handiling. During sttorage at 30℃ for 2 weeks, no increment at all in glucose concentarion was observed in the GA-free unpasteurized sake and the fresh taste and fruity aroma of the original unpasteurized sake were preserved, while in the non-treated sake they deteriorated.Most GA adsorbed on the column was eluted with a rice-liquefied solution, and the recovered GA could be directly re-utilized as a raw material in sake brewing.
- 公益社団法人日本生物工学会の論文
- 1992-08-25
著者
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Hata Yoji
Res. Inst. Gekkeikan Sake Co. Ltd. 101 Shimotoba-koyanagi-cho Fushimi-ku Kyoto 612-8385 Jpn
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Ono Kazuhisa
Department Of Fermentation Technology Faculty Of Engineering Hiroshima University
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Ono Kazuhisa
Faculty Of Engineering Hiroshima University
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Abe Yasuhisa
Res. Inst. Gekkeikan Sake Co. Ltd.
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Abe Yasuhisa
Research Institute For Fundamental Physics Kyoto University
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Hata Yoji
Research Institite Of Gekkeikan Gekkeikan Sake Co. Ltd.
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IMAYASU Satoshi
Research Institite of Gekkeikan, Gekkeikan Sake Co., Ltd.
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OKA Satoru
Faculty of Engineering, Hiroshima University
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Abe Yasushisa
Research Institite Of Gekkeikan Gekkeikan Sake Co. Ltd.
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Kawato A
Research Institute Gekkeikan Sake Co. Ltd.
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Kawato Akitsugu
Research Institute Gekkeikan Sake Co. Ltd.
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Oka S
Faculty Of Engineering Hiroshima University
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Oka Satoru
Department Of Fermentation Technology Faculty Of Engineering Hiroshima University
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Imayasu Satoshi
Research Institute Gekkeikan Sake Co. Ltd.
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Abe Yasuhisa
Research Institute Gekkeikan Sake Co. Ltd.
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Abe Yasuhisa
Research Center For Nuclear Physics Osaka University
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