Expression of a Major House Dust Mite Allergen Gene from Dermatophagoides farinae in Lotus japonicus Accession Miyakojima MG-20 (Plant Biotechnology)
スポンサーリンク
概要
- 論文の詳細を見る
Transformation of a model legume Lotus japonicus accession Miyakojima MG-20 was examined using Agrobacterium tumefaciens with a binary expression vector. Using the improved transformation method, we introduced a major allergen gene from a house dust mite, Der f 1, into MG-20. Analyses by Southern hybridization, reverse transcription (RT)-PCR, and Western blotting showed that the Der f 1 gene was integrated into the genome of L. japonicus, expressing the gene product in the T1 lines. Our results imply future application of oral allergen-specific immunotherapy using legume plants.
- 社団法人日本生物工学会の論文
- 2005-02-25
著者
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ONO Kazuhisa
Department of Molecular Biotechnology, Graduate School of Advanced Sciences of Matter, Hiroshima Uni
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HAYASHI Makoto
Department of Basic Medical Research and Education, Ehime University Graduate School of Medicine
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MUROOKA YOSHIKATSU
Department of Agricultural Chemistry, Faculty of Bioresources, Mie University, Department of Biotech
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Ono Kazuhisa
Department Of Fermentation Technology Faculty Of Engineering Hiroshima University
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Ono Kazuhisa
Department Of Molecular Biotechnology Graduate School Of Advanced Seience Of Matter Hiroshima Univer
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GOTO YUJI
Department of Biotechnology, Institute for Protein Research, Osaka University
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KATO Tomoaki
Department of Surgery, Division of Transplantation, University of Miami School of Medicine
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Goto Yuji
Department Of Biotechnology Graduate School Of Engineering Osaka University
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Murooka Yoshikatsu
Depart. Of Bioteclnology Graduate School Of Eng. Osaka Univ.
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Kato Tomoaki
Department Of Biotechnology Graduate School Of Engineering Osaka University
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Hayashi Makoto
Depart. Of Bioteclnology Graduate School Of Eng. Osaka Univ.
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