cDNA Cloning and Expression of a Gene for Isocitrate Lyase in Pumpkin Cotyledons
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概要
- 論文の詳細を見る
A cDNA clone for isocitrate lyase (ICL) was isolated from a cDNA Iibrary prepared from the poly(A)^ RNA of etiolated pumpkin cotyledons. The cDNA encoded a poly-peptide with 576 amino acids, whose sequence is more than 79% identical to those of ICL from other higher plants and contains the C-terminal tripeptide, Ser-Arg-Met, which is a putative targeting signal to microbodies. Immunogold analysis revealed that ICL protein is exclusively localized in microbodies in etiolated pumpkin cotyledons. Double labeling experiments with protein A-gold particles of different sizes showed that ICL protein coexists with a leaf-peroxisomal enzyme, glycolate oxidase, in the microbodies of greening and senescing pumpkin cotyledons, indicating that transformation between glyoxysomes and leaf peroxisomes occurs directly during greening and senescence of the cotyledons. Immunoblot analysis showed that the amount of ICL protein increased markedly during germination and decreased rapidly when seedlings were exposed to illumination, which induced the microbody transition from glyoxysomes to leaf peroxisomes. In senescing cotyledons, the level of the ICL protein and ICL mRNA increased again with the reverse transition of microbodies from leaf peroxisomes to glyoxysomes. Changes in the amount of ICL protein did not correspond to the changes in the level of ICL mRNA during greening and senescence of the cotyledons, an indication that post-transcriptional regulation plays an important role in the microbody transition.
- 日本植物生理学会の論文
著者
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Mano Shoji
Department of Cell Biology, National Institute for Basic Biology
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NISHIMURA Mikio
Department of Cell Biology, National Institute for Basic Biology
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HAYASHI Makoto
Department of Basic Medical Research and Education, Ehime University Graduate School of Medicine
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Mano Shoji
Dep. Cell Biol. Natl. Inst. Basic Biol.
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Mano Shoji
Department Of Cell Biology National Institute For Basic Biology
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Moriya Kei
Department Of Applied Biology Faculty Of Textile Science And Technology Shinshu University
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Hayashi M
Department Of Cell Biology National Institute For Basic Biology
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Nishimura Mikio
Department Of Cell Biology National Institute For Basic Biology
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KONDO Maki
Department of Cell Biology, National Institute for Basic Biology
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Kojima M
Department Of Bioresource Chemistry Obihiro University Of Agriculture And Veterinary Medicine
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Kojima Michiyuki
Department Of Bioresource Chemistry Obihiro University Of Agriculture And Veterinary Medicine
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Kondo Maki
Department Of Cell Biology National Institute For Basic Biology
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Mano Syoji
Department Of Cell Biology National Institute For Basic Biology
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Komai Michio
Nof Corporation
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Hayashi Makoto
Depart. Of Bioteclnology Graduate School Of Eng. Osaka Univ.
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