Gateway Binary Vectors with the Bialaphos Resistance Gene, bar, as a Selection Marker for Plant Transformation
スポンサーリンク
概要
- 論文の詳細を見る
We constructed two series of Gateway binary vectors, pGWBs and R4pGWBs, possessing the bialaphos resistance gene (bar) as a selection marker for plant transformation. The reporters and tags employed in this system are sGFP, GUS, LUC, EYFP, ECFP, G3GFP, mRFP, TagRFP, 6xHis, FLAG, 3xHA, 4xMyc, 10xMyc, GST, T7 and TAP. Selection of Arabidopsis transformants with BASTA® was successfully carried out using both plate-grown and soil-grown seedlings. Transformed rice calli and suspension-cultured tobacco cells were selected on plates containing BASTA® or glufosinate-ammonium. These vectors are compatible with existing pGWB and R4pGWB vectors carrying kanamycin and hygromycin B resistance.
- 社団法人 日本農芸化学会の論文
- 2010-06-23
著者
-
Nakamura Shinya
Department of Molecular and Functional Genomics, Center for Integrated Research in Science, Shimane
-
Mano Shoji
Department of Cell Biology, National Institute for Basic Biology
-
Tanaka Yuji
Department of Molecular and Functional Genomics, Center for Integrated Research in Science, Shimane
-
OHNISHI Masato
Department of Molecular and Functional Genomics, Center for Integrated Research in Science, Shimane
-
NAKAMORI Chihiro
Department of Cell Biology, National Institute for Basic Biology
-
ARAKI Masami
Department of Cell Biology, National Institute for Basic Biology
-
NIWA Tomoko
Department of Biological Mechanisms and Functions, Graduate School of Bioagricultural Sciences, Nago
-
NISHIMURA Mikio
Department of Cell Biology, National Institute for Basic Biology
-
KAMINAKA Hironori
Department of Bioresource Sciences, Faculty of Agriculture, Tottori University
-
NAKAGAWA Tsuyoshi
Department of Molecular and Functional Genomics, Center for Integrated Research in Science, Shimane
-
SATO Yutaka
Department of Biological Mechanisms and Functions, Graduate School of Bioagricultural Sciences, Nago
-
ISHIGURO Sumie
Department of Biological Mechanisms and Functions, Graduate School of Bioagricultural Sciences, Nago
-
Tanaka Yuji
Department Of Electronics Faculty Of Engineering Nagoya University
-
Ishiguro Sumie
Dep. Of Biological Mechanisms And Functions Graduate School Of Bioagricultural Sciences Nagoya Univ.
-
Ishiguro S
Nagoya Univ. Nagoya Jpn
-
Ishiguro Sumie
Dept. Botany Grad. Sch. Science Kyoto Univ.
-
Ishiguro Sumie
Department Of Biological Mechanisms And Functions Graduate School Of Bioagricultural Sciences Nagoya
-
Takahashi N
Plant Functions Laboratory Riken
-
Mano Shoji
Dep. Cell Biol. Natl. Inst. Basic Biol.
-
Mano Shoji
Department Of Cell Biology National Institute For Basic Biology
-
Niwa Tomoko
Department Of Applied Chemistry Nagoya Institute Of Technology
-
Ohnishi Masato
Department Of Materials Science And Technology Faculty Of Industrial Science And Technology Science
-
Nishimura M
Dep. Of Cell Biology National Inst. For Basic Biology
-
Nishimura Mikio
Department Of Cell Biology National Institute For Basic Biology
-
Nishimura Mikio
Dept. Cell Biol. Nat. Inst. Basic. Biol.
-
Nakano Takeshi
Riken Advanced Sci. Inst.
-
Takahashi Nobutaka
Frontier Research Program The Institute Of Physical And Chemical Research
-
Takahashi Nobutaka
Frontier Research Program The Institute Of Physical And Chemical Research (rlken)
-
Nakamori Chihiro
Dep. Of Cell Biology National Inst. For Basic Biology
-
Nakagawa Tetsuto
Department Of Bioscience And Biotechnology Division Of Bioresource And Bioenvironmental Sciences Gra
-
Nakagawa Tsuyoshi
Dep. Of Molecular And Functional Genomics Center For Integrated Res. In Sci. Shimane Univ.
-
NAKANO Takeshi
The Institute of Physical and Chemical Research (RIKEN)
-
Nishimura Mikio
Dep. Of Cell Biology National Inst. For Basic Biology
-
Nakagawa T
Department Of Bioscience And Biotechnology Division Of Bioresource And Bioenvironmental Sciences Gra
-
Nakagawa Tsuyoshi
Department Of Molecular And Functional Genomics Center For Integrated Research In Science Shimane Un
-
Nishimura Mikio
National Inst. For Basic Biology Okazaki Jpn
-
Nakamura Shinya
Department Of Molecular And Functional Genomics Center For Integrated Research In Science Shimane Un
-
Araki Masami
Department Of Cell Biology National Institute For Basic Biology
-
Nakamori Chihiro
Department Of Cell Biology National Institute For Basic Biology
-
Niwa Tomoko
Department Of Biological Mechanisms And Functions Graduate School Of Bioagricultural Sciences Nagoya
-
Mano Syoji
Department Of Cell Biology National Institute For Basic Biology
-
Takahashi Nobuyuki
Hokkaido University Of Education
-
Sato Yutaka
Department Of Biological Mechanisms And Functions Graduate School Of Bioagricultural Sciences Nagoya
-
Kaminaka Hironori
Department Of Bioresource Sciences Faculty Of Agriculture Tottori University
-
Ohnishi Masato
Department Of Molecular And Functional Genomics Center For Integrated Research In Science Shimane Un
-
Ohnishi Masato
Department Of Cardiovascular And Respiratory Medicine Shiga University Of Medical Science
-
Tanaka Yuji
Department Of Molecular And Functional Genomics Center For Integrated Research In Science Shimane Un
-
Nakano Takeshi
Plant Functions Lab. The Inst. Of Physical And Chemical Res.
-
Nakano Takeshi
Plant Functions Lab.
-
Nakamura Shinya
Department Of Clinical Pharmaceutical Science Faculty Of Pharmaceutical Sciences Okayama University
-
Nakamura Shinya
Department Of Chemistry University Of Tsukuba
-
Sato Yutaka
Department Of Biochemistry And Oral Health Science Center Tokyo Dental College
-
Tanaka Yuji
Department Of Applied Chemistry Faculty Of Engineering Kyushu Institute Of Technology
-
Nakagawa Tsuyoshi
Department Of Molecular And Functional Genomics Center For Integrated Research In Science Shimane Un
-
Takahashi Nobuyuki
Laboratory For Molecular Function Of Food Division Of Food Science And Biotechnology Graduate School
-
Takahashi Noriko
Department of Molecular and Functional Genomics, Center for Integrated Research in Science, Shimane University
-
Nakamura Shinya
Department of Molecular and Functional Genomics, Center for Integrated Research in Science, Shimane University
-
Sato Yutaka
Department of Applied Physics, Graduate School of Engineering, Tokyo University of Agriculture and Technology, 2-24-16 Nakacho, Koganei, Tokyo 184-8588, Japan
-
NAKAGAWA Tsuyoshi
Department of Materials Science and Engineering, Graduate School of Engineering, Nagoya Institute of Technology
関連論文
- Gateway Binary Vectors with the Bialaphos Resistance Gene, bar, as a Selection Marker for Plant Transformation
- Cut-Offs for Screening Prolonged QT Intervals From Fridericias Formula in Children and Adolescents
- Markedly Ring-enhanced Optic Nerves Due to Metastasis of Signet-ring Cell Gastric Carcinoma
- Serial Monitoring of Basal Metabolic Rate for Therapeutic Evaluation in an Isaacs Syndrome Patient with Chronic Fluctuating Symptoms
- Longitudinal Analysis of Cytokines and Chemokines in the Cerebrospinal Fluid of a Patient with Neuro-Sweet Disease Presenting with Recurrent Encephalomeningitis
- Dendritic cell immunotherapy for patients with metastatic renal cell carcinoma : University of Tokyo experience
- Cut-Offs for Screening Prolonged QT Intervals From Fridericia's Formula in Children and Adolescents
- Cytosolic Aconitase Participates in the Glyoxylate Cycle in Etiolated Pumpkin Cotyledons : GENE STRUCTURE AND EXPRESSION : PROTEINS, ENZYMES AND METABOLISM
- Spatial Patterns of Sucrose-Inducible and Polygalacturonic Acid-Inducible Expression of Genes That Encode Sporamin and β-Amylase in Sweet Potato : GENE STRUCTURE AND EXPRESSION
- Isolation of the Gene for EILP, an Elicitor-Inducible LRR Receptor-Like Protein, from Tobacco by Differential Display
- Molecular Cloning of a Defense-Response-Related Cytochrome P450 Gene from Tobacco
- The Involvement of Tonoplast Proton Pumps and Na^+(K^+)/H^+ Exchangers in the Change of Petal Color During Flower Opening of Morning Glory, Ipomoea tricolor cv. Heavenly Blue
- PE-413 Association Between the Accumulation of Cardiovascular Risk Factors and Adipokine or Individual Risk Factor Levels in Adolescent Volunteers(PE069,Preventive Medicine/Epidemiology/Education 2 (H),Poster Session (English),The 73rd Annual Scientific M
- Association Between the Number of Cardiovascular Risk Factors and Each Risk Factor Level in Elementary School Children
- Prevalence and Time of Appearance of Brugada Electrocardiographic Pattern in Young Male Adolescents From a 3-year Follow-up Study (Arrhythmia, Diagnosis/Pathophysiology/EPS 8 (A), The 69th Annual Scientific Meeting of the Japanese Circulation Society)
- OJ-260 Prevalence and Age of Appearance of the Brugada Type Electrocardiogram in Young Adolescents(ECG/Body Surface Potential Mapping/Holter 3 (A) : OJ30)(Oral Presentation (Japanese))
- EXPONENTIAL CORRECTION OF QT INTERVAL TO MINIMIZE THE EFFECT OF THE HEART RATE IN CHILDREN
- A Proteinase-Storing Body that Prepares for Cell Death or Stresses in the Epidermal Cells of Arabidopsis
- A NOVEL LARGE BODY THAT IS DERIVED FROM ENDOPLASMIC RETICULUM
- Sodium Bicarbonate Infusion Test : A New Method for Evaluating Parathyroid Function
- β-Glucosidase in the Indigo Plant : Intracellular Localization and Tissue Specific Expression in Leaves
- Heterologous Expression and Subcellular Localization of Pumpkin Seed Tonoplast Intrinsic Proteins (TIP) in Yeast Cells
- Usefulness of novel imaging modalities in diagnosis of focal nodular hyperplasia of the liver
- Detection and Characterization of a Vacuolar Protein(VP24) in Anthocyanin Producing Cells of Sweet Potato in Suspension Culture : PROTEINS, ENZYMES AND METABOLISM : STRUCTURE AND FUNCTION OF CELLS
- AtVAM3 is Required for Normal Specification of Idioblasts, Myrosin Cells
- An ER-Localized Form of PV72, a Seed-Specific Vacuolar Sorting Receptor, Interferes the Transport of an NPIR-Containing Proteinase in Arabidopsis Leaves
- Functional Differentiation of Peroxisomes Revealed by Expression Profiles of Peroxisomal Genes in Arabidopsis thaliana
- The ER Body, a Novel Endoplasmic Reticulum-Derived Structure in Arabidopsis
- A Vacuolar Sorting Receptor PV72 on the Membrane of Vesicles that Accumulate Precursors of Seed Storage Proteins (PAC Vesicles)
- Proteomic Analysis of Leaf Peroxisomal proteins in Greening Cotyledons of Arabidopsis thaliana
- Direct Interaction and Determination of Binding Domains among Peroxisomal Import Factors in Arabidopsis thaliana
- Distribution and Characterization of Peroxisomes in Arabidopsis by Visualization with GFP : Dynamic Morphology and Actin-Dependent Movement
- Developmental Analysis of a Putative ATP/ADP Carrier Protein Localized on Glyoxysomal Membranes During the Peroxisome Transition in Pumpkin Cotyledons
- Distribution and characterization of peroxisomes in Arabidopsis thaliana by visualization with GFP
- Peroxisomal Localization of a Myosin XI Isoform in Arabidopsis thaliana
- Rapid Adsorption and Desorption of Fluoride on Zirconium(IV) Complex with Hydrophilic Chelating Polymer
- Two-Photon Absorption in Tin Dioxide Single Crystal at 532nm
- Transition of children with membranoproliferative glomerulonephritis to adolescence and adulthood
- Two cases of congenital nephrotic syndrome
- Long-Term Follow-up of Renal Transplantation in Children: A 20-Year Experience at One Kidney Center in Japan
- Adipokines and the Prediction of the Accumulation of Cardiovascular Risk Factors or the Presence of Metabolic Syndrome in Elementary School Children
- Wakame Seaweed Suppresses the Proliferation of 7,12-Dimethylhenz(α)-anthracene-induced Mammary Tumors in Rats
- Asymmetric Sidebands of Low Frequency Waves in a Current Carrying Plasma
- Excitation and Damping of Continuous Pseudo Acoustic and Ion Waves in a Plasma
- Ballistic Phase Mixing of Continuous Pseudo Waves in a Plasma
- Ion Wave Damping in a Nonuniform Plasma
- Ion Wave Damping in Nonuniform Plasma
- Proteome Analysis Reveals the Significant Alternations of Cardiac Small Heat Shock Proteins Expression in Heart Failure (Heart Failure, Basic 6 (M), The 69th Annual Scientific Meeting of the Japanese Circulation Society)
- Contribution of Vascular NAD(P)H Oxidase to Endothelial Dysfunction in Heart Failure and the Therapeutic Effects of HMG-CoA Reductase Inhibitor
- PJ-509 Transcardiac Difference of Aldosterone in Patients with Heart Disease(Heart Failure, Clinical 13 (M) : PJ85)(Poster Session (Japanese))
- PJ-418 Relationship Between Transcardiac Increase of C-Reactive Protein and Transcardiac Increase of Interleukin-6 in Patients with Dilated Cardiomyopathy(Cardiomyopathy, Clinical 4 (M) : PJ70)(Poster Session (Japanese))
- PJ-224 The Activity and Expression of Myocardial NADPH Oxidase Are Increased in Heart Failure.(Heart Failure, Basic 2 (M) : PJ38)(Poster Session (Japanese))
- OJ-440 Proteome analysis of myocardial protein expression in heart failure(Heart Failure, Basic 3 (M) : OJ54)(Oral Presentation (Japanese))
- OJ-225 Relationship Between Heart-type Fatty Acid-binding Protein and Left Ventricular Remodeling in Patients with Dilated Cardiomyopathy(Cardiomyopathy, Clinical 1 (M) : OJ26)(Oral Presentation (Japanese))
- FRS-191 Comparison of BMP and N terminal proBNP in Patients with Heart Failure(Heart Failure : Basic and Clinical Viewpoint (M) : FRS22)(Featured Research Session (English))
- Neurohumoral Effects of Long Term Infusion of hANP in Patients with Severe Congestive Heart Failure
- Nutrient limitation of the primary production of phytoplankton in Lake Baikal
- Characterization of Organelles in the Vacuolar-Sorting Pathway by Visualization with GFP in Tobacco BY-2 Cells
- Microbody Defective Mutants of Arabidopsis
- Hydroxypyruvate Reductase with a Carboxy-Terminal Targeting Signal to Microbodies is Expressed in Arabidopsis
- cDNA Cloning and Expression of a Gene for Isocitrate Lyase in Pumpkin Cotyledons
- Functional Transformation of Microbodies in Higher Plant Cells
- Development of Gateway Binary Vectors, R4L1pGWBs, for Promoter Analysis in Higher Plants
- Development of R4 Gateway Binary Vectors (R4pGWB) Enabling High-Throughput Promoter Swapping for Plant Research
- HBME-1 Immunostaining in Thyroid Tumors Especially in Follicular Neoplasm
- Successful Surgical Treatment of a Solitary Parapharyngeal Metastasis from Thyroid Cancer, Using the Mandibular Swing-Transcervical Approach: Report of a Case
- Incidentally Discovered Adrenocortical Adenomas Are Not Fully Nonfunctioning: lmmunohistochemical and Dispersed Adrenocortical Cell Study
- Differentiation between N-Acetylchondrosine and N-Acetyldermosine by Matrix-Assisted Laser Desorption Ionization Time of Flight Post Source Decay Mass Spectrometry
- Modulation of organic anion transporting polypeptide 1 and multidrug resistance protein 3 expression in the liver and kidney of Gunn rats
- Synthesis and Characterization of Collagenous Peptides Cross-Linked at Their Amino and Carboxy Termini
- Effects of Shear Stress on the Self-Assembly Process of Elastomeric Polypeptides
- Synthesis and Characterization of Peptide-Surfactants Using Elastin Repeating Unit
- Fluorescence Energy Transfer to Tailed Acridine Oranges from Pyrene Excimer in Four α-Helix Bundle 53-peptide
- Construction of Four α-Helix Bundle Structure with L- and D-Amino Acid Segments
- The Building of Antiparallel Four α-Helix Bundle Structure with a Pair of Pyrenylalanines on a Cyclic Pseudo-peptide Template
- 脳性麻痺による長期臥床を余儀なくされた続発性骨粗鬆症の現状
- Screening of a Mutant Plasmid with High Expression Efficiency of GC-Rich leuB Gene of an Extreme Thermophile, Thermus thermophilus, in Escherichia coli
- Long-term follow-up of type II membranoproliferative glomerulonephritis in two children
- Equivalent Circuit Model and Magnetic Field Diffusion Equation in the Tokamak Simulation
- Equilibrium in a Multipole Tokamak
- An HTLV-I carrier with Graves' disease followed by uveitis: isolation of HTLV-I from thyroid tissue
- Low-Threshold Photopumped Distributed Feedback Plastic Laser Made by Replica Molding
- Photopumped Organic Solid-State Dye Laser with a Second-Order Distributed Feedback Cavity : Optics and Quantum Electronics
- Transcardiac Gradient of Plasma Heart-Type Fatty Acid Binding Protein as a Maker of Myocardial Injury in Patients with Dilated Cardiomyopathy
- Lipidation of the Collagen Model Peptide to Make Peptide-Surfactant
- Two Types of Cation Binding Sites on Elastomeric Polypeptides : Effects on Self-Assembly Process and Elastomeric Functions
- New apparatuses for cultivation of appendicularians
- Design of the Triple-Stranded Coiled-Coil Structure Incorporating Ion Pair Residues in the Hydrophobic Core
- Suppression of Peroxisome Biogenesis Factor 10 Reduces Cuticular Wax Accumulation by Disrupting the ER Network in Arabidopsis thaliana
- Plant Catalase is Imported into Peroxisomes by Pex5p but is Distinct from Typical PTS1 Import
- Endogenous Bradykinin Suppresses Myocardial Fibrosis through the Cardiac Generated Endothelin System under Chronic ACE Inhibition in Heart Failure
- Relationship between Plasma Level of Cardiotrophin-1 and Prognosis in Patients with Dilated Cardiomyopathy
- Expression of Somatostatin Receptor and Effects of Somatostatin Analog on Pancreatic Endocrine Tumors
- Development of R4 Gateway Binary Vectors (R4pGWB) Enabling High-Throughput Promoter Swapping for Plant Research
- Improved Gateway Binary Vectors : High-Performance Vectors for Creation of Fusion Constructs in Transgenic Analysis of Plants
- LIPO-MICRODIALYSIS : A NEW MICRODIALYSIS METHOD FOR STUDYING THE PHARMACOKINETICS OF LIPOPHILIC SUBSTANCES
- Seeing Is Believing : On the Use of Image Databases for Visually Exploring Plant Organelle Dynamics
- Development of Gateway Binary Vectors, R4L1pGWBs, for Promoter Analysis in Higher Plants
- Gateway Binary Vectors with the Bialaphos Resistance Gene, bar, as a Selection Marker for Plant Transformation
- A Defect of Peroxisomal Membrane Protein 38 Causes Enlargement of Peroxisomes