Development of R4 Gateway Binary Vectors (R4pGWB) Enabling High-Throughput Promoter Swapping for Plant Research
スポンサーリンク
概要
- 論文の詳細を見る
We developed a new series of Gateway binary vectors, R4pGWBs, that are plant transformation vectors designed for one-step construction of chimeric genes between any promoter and any cDNA. The structure of R4pGWBs is almost the same as the promoterless type of improved pGWBs (ImpGWBs), except that the attR1 site is replaced with attR4, which enables tripartite recombination of these vectors with promoter- and cDNA-entry clones. While ImpGWBs are suitable for promoter analysis and constitutive expression of cDNAs in higher plants, R4pGWBs have a great advantage in expressing a cDNA under the regulation of desired promoters.
- 社団法人 日本農芸化学会の論文
著者
-
Nakamura Shinya
Department of Molecular and Functional Genomics, Center for Integrated Research in Science, Shimane
-
NAKAGAWA Tsuyoshi
Department of Molecular and Functional Genomics, Center for Integrated Research in Science, Shimane
-
ISHIGURO Sumie
Department of Biological Mechanisms and Functions, Graduate School of Bioagricultural Sciences, Nago
-
Suzuki Takamasa
Department Of Electronics Nagoya University:r&d Department Nippondenso Co. Ltd.
-
Tanaka K
Institute Of Molecular And Cellular Biosciences The University Of Tokyo
-
KAWAMUKAI Makoto
Department of Applied Bioscience and Biotechnology, Faculty of Life and Environmental Science
-
Tanaka Katsunori
Department of Applied Bioscience and Biotechnology, Faculty of Life and Environmental Science, Shima
-
Tanaka K
Tottori Univ. Tottori
-
Kimura Tetsuya
Department Of Sustainable Resource Science Graduate School Of Of Bioresources Mie University
-
NAKAMURA Kenzo
Department of Urology, University of Yamanashi
関連論文
- Gateway Binary Vectors with the Bialaphos Resistance Gene, bar, as a Selection Marker for Plant Transformation
- Strontium Isotope Effects Observed in Liquid Chromatography with Crown Ether Resins
- A Study on Zinc Isotope Fractionation in a Benzo Crown Resin/Acetone System
- Zinc Isotope Accumulation in Liquid Chromatography with Crown Ether Resin
- Novel Syntheses Method of Phenol Type Benzo-15-Crown-5 Ether Resin and its Application for Lithium Isotope Separation
- Zinc Isotope Separation by Phenol Formaldehyde Type 15-Crown-5 Resin in Organic Solvents
- Analysis of the Decaprenyl Diphosphate Synthase (dps) Gene in Fission Yeast Suggests a Role of Ubiquinone as an Antioxidant^1
- Cloning and Functional Expression of a Novel Geranylgeranyl Pyrophosphate Synthase Gene from Arabidopsis thaliana in Escherichia coli
- Production of Ubiquinone in Escherichia coli by Expression of Various Genes Responsible for Ubiquinone Biosynthesis
- Identification and Molecular Analysis of Lactococcus lactis rpoD Operon
- Genetic and Molecular Analysis of the rpoD Gene from Lactococcus lactis
- Cell-Cycle Dependent Dynamic Change of 26S Proteasome Distribution in Tobacco BY-2 Cells
- Development of series of gateway binary vectors, pGWBs, for realizing efficient construction of fusion genes for plant transformation
- -P85- RELATION BETWEEN TL-201 MYOCARDIAL SPECT FINDINGS AND PROGNOSIS IN DILATED CARDIOMYOPATHY : RI : FREE COMMUNICATIONS(I) : PROCEEDINGS OF THE 51th ANNUAL SCIENTIFIC MEETING OF THE JAPANESE CIRCULATION SOCIETY
- Identification and Subcellular Localization of Two Solanesyl Diphosphate Synthases from Arabidopsis thaliana
- Effects of Dietary Oyster Extract on Lipid Metabolism, Blood Pressure, and Blood Glucose in SD Rats, Hypertensive Rats, and Diabetic Rats
- Hypotriacylglycerolemic and Antiobesity Properties of a New Fermented Tea Product Obtained by Tea-Rolling Processing of Third-Crop Green Tea (Camellia sinensis) Leaves and Loquat (Eriobotrya japonica) Leaves
- Hypoglycemic Activity of Eriobotrya japonica Seeds in Type 2 Diabetic Rats and Mice
- Effects of Feeding Oyster, Crassostrea gigas, on Serum and Liver Lipid Levels in Rats
- Ultrastructure of Mature Protein Body in the Starchy Endosperm of Dry Cereal Grain
- Highly efficient production of human interferon-α by transgenic cultured rice cells
- Production of biologically active human interferon-α in transgenic rice
- A Novel Vesicle Derived Directly from Endoplasmic Reticulum is Involved in the Transport of Vacuolar Storage Proteins in Rice Endosperm
- Differential Localization of Tonoplast Intrinsic Proteins on the Membrane of Protein Body Type II and Aleurone Grain in Rice Seeds
- Preparation of an Endosperm Protoplasts from a Dwarf Rice Variety and Transient Expression of Green-Fluorescent Protein
- Separation of Specific Cells Using a Laser Micro-dissection System and Detection of mRNA in Developing Rice Endosperm Tissue
- Hosetsu-dwarf ; Useful Rice for Molecular Biological Research on Plant Seed Development
- Gateway vectors for plant transformation
- Transition of children with membranoproliferative glomerulonephritis to adolescence and adulthood
- Two cases of congenital nephrotic syndrome
- Long-Term Follow-up of Renal Transplantation in Children: A 20-Year Experience at One Kidney Center in Japan
- FOURIER ANALYSIS OF MULTI-GATED BLOOD-POOL DATA IN PATIENTS WITH CONGENITAL HEART DISEASE : PROCEEDINGS OF THE 47th ANNUAL SCIENTIFIC MEETING OF THE JAPANESE CIRCULATION SOCIETY : Radioisotope
- EVALUATION OF VENTRICULAR CONTRACTION ABNORMALITY USING MULTIPLE GATED BLOOD POOL SCINTIGRAPHY AND ITS FOURIER ANALYSIS : Radio isotope : FREE COMMUNICATIONS (Abstract) : 45 Annual Scientific Meeting, Japanese Circulation Society
- Regulation of CAPRICE Transcription by MYB Proteins for Root Epidermis Differentiation in Arabidopsis
- The analysis of CPC gene in root hair formation in Arabidopsis thaliana
- Regulatory Systems of Root Patterning
- Enhanced Piezoelectric Property of Barium Titanate Single Crystals with Engineered Domain Configurations
- Change of Macroscopic and Microscopic Symmetry of Barium Titanate Single Crystal around Curie Temperature
- Measurement of Semiconductor Heterojunction Band Discontinuities Using Free Electron Laser
- A Cytokinin-Repressed Gene in Cucumber for a bHLH Protein Homologue is Regulated by Light
- Development of Gateway Binary Vectors, R4L1pGWBs, for Promoter Analysis in Higher Plants
- Development of R4 Gateway Binary Vectors (R4pGWB) Enabling High-Throughput Promoter Swapping for Plant Research
- Enhancement of Electron Mobility in Quasi-One-Dimensional Structure
- Comparative Study of C-V and Transconductance of a Si δ-Doped GaAs FET Structure
- Picosecond Photoluminescence Study of Relaxation Phenomena of Hot Electrons in a Quasi-One-Dimensional Structure
- On the Electron Mobility in Quasi-One-Dimensional Structures Fabricated by Holographic Lithography and Wet Chemical Etching
- Application of HfSiON to Deep Trench Capacitors of Sub-45nm Node Embedded DRAM
- Quantitative Analysis of Domain Walls in Magneto-Optic Thin Films By Magnetic Force Microscopy
- Optical Parametric Amplification using the Phase Matching Retracing Behavior in MgO:LiNbO_3 for Generation of Intense Widely Tunable Mid-infrared Pulses
- Synthesis of Diamond by DC Plasrna Chemical Vapor Deposition above the Surface of a Water-Ethylene Glycol Solution
- 酸化スズガスセンサ膜の微構造およびホ-ル効果の膜厚依存
- The Effect of the Particle Sizes and the Correlational Sizes of Dipoles Introduced by the Lattice Defects on the Crystal Structure of Barium Titanate Fine Particles
- 29a-YB-3 An intense picosecond mid-infrared light source and its application to sum frequency generation from surfaces
- Defect Structure of Heteroepitaxial SnO_2 Thin Films Grown on TiO_2 Substrates : Surfaces, Interfaces, and Films
- Local Epitaxial Growth of (103) One-Axis-Oriented SrBi_2Ta_2O_9 Thin Films Prepared at Low Deposition Temperature by Metalorganic Chemical Vapor Deposition and Their Electrical Properties
- Effect of Nonstoichiometry on Microstructure of Epitaxially Grown BaTiO_3 Thin Films
- Thermal Stability of Artificial SrTiO_3/SrO Superlattice Epitaxially Grown on SrTiO_3 Single Crystal
- Solid Solution of Epitaxially Grown(Sr, Mg)TiO_3 Thin Films
- Electrical Characterization of SrTiO_3 Thin Films Grown on Nb-Doped SrTiO_3 Single Crystals
- Artificial SrTiO_3/SrO Superlattices by Pulsed Laser Deposition
- Interface and Domain Structures of (116)-Oriented SrBi_2Ta_2O_9 Thin Film Epitaxially Grown on (110) SrTiO_3 Single Crystal
- Interface and Defect Structures of (001)-Oriented SrBi_2Ta_2O_9 Thin Film Epitaxially Grown on (001) SrTiO_3 Single Crystal
- Optical Confinement Effect for below 5 μm Thin Film Poly-Si Solar Cell on Glass Substrate
- Cloning and Sequencing Analysis of Alginate Lyase Genes from the Marine Bacterium Vibrio sp. O2
- Effects of Fucoidan from Mozuku on Human Stomach Cell Lines
- Synthesis of Novel 26-Substituted Milbemycin A_4 Derivatives and Their Acaricidal Activities
- Genetic Analysis of chs1^+ and chs2^+ Encoding Chitin Synthases from Schizosaccharomyces pombe
- Interaction between a Negative Regulator (Msa2/Nrd1) and a Positive Regulator (Cpc2) of Sexual Differentiation in Schizosaccharomyces pombe
- Truncated Sla1 Induces Haploid Meiosis through the Path1-Mei2 System in Fission Yeast
- Synthesis and Insecticidal Activity of Novel N-Oxydihydropyrrole Derivatives with a Substituted Spirocyclohexyl Group(Organic Chemistry)
- Synthesis of Milbemycins α_9, α_, α_, α_, α_, α_, α_, α_, α_, α_, α_, α_, Δ^, Δ^-Milbemycins A_3, A_4, from Milbemycins A_3, A_4, and Their Acaricidal Activities
- Synthesis and Insecticidal Activity of N-Oxydihydropyrroles : 4-Hydroxy-3-mesityl-5, 5-dimethyl Derivatives with Various Substituents at the 1-Position(Organic Chemistry)
- 脳性麻痺による長期臥床を余儀なくされた続発性骨粗鬆症の現状
- Regio- and Stereochemical Study of Sex Pheromone of Pine Sawfly; Diprion nipponica
- Long-term follow-up of type II membranoproliferative glomerulonephritis in two children
- Loss of AtPDR8, a Plasma Membrane ABC Transporter of Arabidopsis thaliana, Causes Hypersensitive Cell Death Upon Pathogen Infection
- Zinc Transporter of Arabidopsis thaliana AtMTP1 is Localized to Vacuolar Membranes and Implicated in Zinc Homeostasis
- Quadrupolar Effect in Strongly Correlated Systems
- Two Glutamic Acids in Chitosanase A from Matsuebacter chitosanotabidus 3001 Are the Catalytically Important Residues
- The Arabidopsis FLAKY POLLEN1 Gene Encodes a 3-Hydroxy-3-Methylglutaryl-Coenzyme A Synthase Required for Development of Tapetum-Specific Organelles and Fertility of Pollen Grains
- Identification of kaonashi Mutants Showing Abnormal Pollen Exine Structure in Arabidopsis thaliana
- Arabidopsis AUXIN RESPONSE FACTOR6 and 8 Regulate Jasmonic Acid Biosynthesis and Floral Organ Development via Repression of Class 1 KNOX Genes
- Development of R4 Gateway Binary Vectors (R4pGWB) Enabling High-Throughput Promoter Swapping for Plant Research
- Improved Gateway Binary Vectors : High-Performance Vectors for Creation of Fusion Constructs in Transgenic Analysis of Plants
- Phosphocholine-Containing Glycosyl Inositol-Phosphoceramides from Trichoderma viride Induce Defense Responses in Cultured Rice Cells
- LIPO-MICRODIALYSIS : A NEW MICRODIALYSIS METHOD FOR STUDYING THE PHARMACOKINETICS OF LIPOPHILIC SUBSTANCES
- Hyperproduction of 3,4-Dihydroxyphenyl-L-alanine (L-Dopa) Using Erwinia herbicola Cells Carrying a Mutant Transcriptional Regulator TyrR
- A Rapid, Simple, and Effective Method of Constructing a Randomly Mutagenized Plasmid Library Free from Ligation
- Differential Expression Control and Polarized Distribution of Plasma Membrane-Resident SYP1 SNAREs in Arabidopsis thaliana
- Development of Gateway Binary Vectors, R4L1pGWBs, for Promoter Analysis in Higher Plants
- Hypoglycemic Activity of Eriobotrya japonica Seeds in Type 2 Diabetic Rats and Mice
- Ultrastructure of Mature Protein Body in the Starchy Endosperm of Dry Cereal Grain
- Thin Frozen Film Method for Visualization of Storage Proteins in Mature Rice Grains
- Gateway Binary Vectors with the Bialaphos Resistance Gene, bar, as a Selection Marker for Plant Transformation
- MAA3 (MAGATAMA3) Helicase Gene is Required for Female Gametophyte Development and Pollen Tube Guidance in Arabidopsis thaliana
- Calcium isotope fractionation in liquid chromatography with crown ether resins
- Kluyveromyces marxianus-based platform for direct ethanol fermentation and recovery from cellulosic materials under air-ventilated conditions(MICROBIAL PHYSIOLOGY AND BIOTECHNOLOGY)
- Changes in Microbiota Population during Fermentation of Narezushi as Revealed by Pyrosequencing Analysis
- Differences in intron-mediated enhancement of gene expression by the first intron of cytosolic superoxide dismutase gene from rice in monocot and dicot plants
- Kluyveromyces marxianus-based platform for direct ethanol fermentation and recovery from cellulosic materials under air-ventilated conditions