Improved Gateway Binary Vectors : High-Performance Vectors for Creation of Fusion Constructs in Transgenic Analysis of Plants
スポンサーリンク
概要
- 論文の詳細を見る
We made a series of improved Gateway binary vectors (pGWBs) for plant transformation. Fifteen different reporters and tags, sGFP, GUS, LUC, EYFP, ECFP, G3GFP, mRFP, 6×His, FLAG, 3×HA, 4×Myc, 10×Myc, GST, T7, and TAP, were employed. Some vectors carry the 2×35S-Ω promoter for higher-level expression. The kanamycin- and hygromycin-resistant markers are independently available for each of the 43 types of vectors, thus an additional transformation of once-transformed plants can be carried out easily. Their small size and high-copy number in Escherichia coli make possible easier handling at plasmid preparation and sequencing. Improved pGWBs should be a powerful tool for transgenic research in plants.
- 社団法人 日本農芸化学会の論文
- 2007-08-23
著者
-
Nakamura Shinya
Department of Molecular and Functional Genomics, Center for Integrated Research in Science, Shimane
-
NAKAGAWA Tsuyoshi
Department of Molecular and Functional Genomics, Center for Integrated Research in Science, Shimane
-
ISHIGURO Sumie
Department of Biological Mechanisms and Functions, Graduate School of Bioagricultural Sciences, Nago
-
Niwa Yasuo
Plant Science Center Riken(the Institute Of Physical And Chemical Research)
-
Niwa Yasuo
Lab. Of Plant Cell Technol. Graduate School Of Nutritional And Environmental Sciences Univ. Of Shizu
-
Niwa Yasuo
Laboratory Of Plant Cell Technology And Coe Program In The 21st Century Graduate School Of Nutrition
-
Watanabe Yuichiro
Department of Life Science, Graduate School of Arts and Sciences, University of Tokyo
-
Suzuki Takamasa
Department Of Electronics Nagoya University:r&d Department Nippondenso Co. Ltd.
-
Ishiguro Sumie
Dep. Of Biological Mechanisms And Functions Graduate School Of Bioagricultural Sciences Nagoya Univ.
-
Ishiguro S
Nagoya Univ. Nagoya Jpn
-
Ishiguro Sumie
Dept. Botany Grad. Sch. Science Kyoto Univ.
-
Ishiguro Sumie
Department Of Biological Mechanisms And Functions Graduate School Of Bioagricultural Sciences Nagoya
-
Takahashi N
Plant Functions Laboratory Riken
-
Tanaka K
Institute Of Molecular And Cellular Biosciences The University Of Tokyo
-
Tanaka Katsunori
Department of Applied Bioscience and Biotechnology, Faculty of Life and Environmental Science, Shima
-
Tanaka K
Tottori Univ. Tottori
-
Nakamura K
Nagoya Univ. Nagoya Jpn
-
Hino Takeshi
Department of Molecular and Functional Genomics, Center for Integrated Research in Science, Shimane
-
Nakano Takeshi
Riken Advanced Sci. Inst.
-
Kimura Tetsuya
Department Of Sustainable Resource Science Graduate School Of Of Bioresources Mie University
-
NAKAMURA Kenzo
Department of Urology, University of Yamanashi
-
Takahashi Nobutaka
Frontier Research Program The Institute Of Physical And Chemical Research
-
Takahashi Nobutaka
Frontier Research Program The Institute Of Physical And Chemical Research (rlken)
-
Nakagawa Tetsuto
Department Of Bioscience And Biotechnology Division Of Bioresource And Bioenvironmental Sciences Gra
-
Nakagawa Tsuyoshi
Dep. Of Molecular And Functional Genomics Center For Integrated Res. In Sci. Shimane Univ.
-
Tanaka Kiyoshi
Coll. Environ. Health Azabu Univ.
-
NAKANO Takeshi
The Institute of Physical and Chemical Research (RIKEN)
-
Kimura Tetsuya
Department Of Clinical Retrovirology And Infectious Diseases Center For Aids Research Kumamoto Unive
-
Hino Takeshi
Department Of Molecular And Functional Genomics Center For Integrated Research In Science Shimane Un
-
Kimura Tetsuya
Dep. Of Sustainable Resource Sci. Graduate School Of Bioresources Mie Univ.
-
Nakagawa T
Department Of Bioscience And Biotechnology Division Of Bioresource And Bioenvironmental Sciences Gra
-
Nakagawa Tsuyoshi
Department Of Molecular And Functional Genomics Center For Integrated Research In Science Shimane Un
-
Nakamura Kenzo
Department Of Biological Mechanisms And Functions Graduate School Of Bioagricultural Sciences Nagoya
-
Suzuki Toshiya
Department Of Biological Mechanisms And Functions Graduate School Of Bioagricultural Sciences Nagoya
-
TABATA Ryo
Department of Biological Mechanisms and Functions, Graduate School of Bioagricultural Sciences, Nago
-
MURATA Satoko
Department of Biological Functions and Mechanisms Graduate School of Bioagricultural Sciences, Nagoy
-
MAEO Kenichiro
Department of Biological Functions and Mechanisms Graduate School of Bioagricultural Sciences, Nagoy
-
KAWAI Tsutae
Department of Biological Functions and Mechanisms Graduate School of Bioagricultural Sciences, Nagoy
-
Nakamura Shinya
Department Of Molecular And Functional Genomics Center For Integrated Research In Science Shimane Un
-
Takahashi Nobuyuki
Hokkaido University Of Education
-
Tabata Ryo
Department Of Biological Mechanisms And Functions Graduate School Of Bioagricultural Sciences Nagoya
-
Maeo Kenichiro
Department Of Biological Functions And Mechanisms Graduate School Of Bioagricultural Sciences Nagoya
-
Kawai Tsutae
Department Of Biological Functions And Mechanisms Graduate School Of Bioagricultural Sciences Nagoya
-
Murata Satoko
Department Of Biological Functions And Mechanisms Graduate School Of Bioagricultural Sciences Nagoya
-
Watanabe Yuichiro
Dep. Of Life Sci. Graduate School Of Arts And Sci. The Univ. Of Tokyo
-
Nakano Takeshi
Plant Functions Lab. The Inst. Of Physical And Chemical Res.
-
Nakano Takeshi
Plant Functions Lab.
-
Nakamura Shinya
Department Of Clinical Pharmaceutical Science Faculty Of Pharmaceutical Sciences Okayama University
-
Kikuchi Takao
Coll. Environ. Health Azabu Univ.
-
Nakamura Shinya
Department Of Chemistry University Of Tsukuba
-
Nakamura Kenzo
Department Of Applied Biological Sciences Faculty Of Agricultural Sciences Nagoya University
-
Tanaka Keiko
Department Of Food Science And Technology Faculty Of Agriculture Kyoto University
-
Watanabe Yuichiro
Department Of Life Science Graduate School Of Arts And Science The University Of Tokyo
-
Nakagawa Tsuyoshi
Department Of Molecular And Functional Genomics Center For Integrated Research In Science Shimane Un
-
Suzuki Takamasa
Department Of Applied Science Faculty Of Engineering Kyushu University
-
Takahashi Nobuyuki
Laboratory For Molecular Function Of Food Division Of Food Science And Biotechnology Graduate School
-
Koyanagi Takashi
Research Institute For Bioresources And Biotechnology Ishikawa Prefectural University
-
Suzuki Takamasa
Department Of Biological Functions And Mechanisms Graduate School Of Bioagricultural Sciences Nagoya University
-
Kimura Tetsuya
Department of Biochemistry, Faculty of Pharmaceutical Sciences, Niigata University of Pharmacy and Applied Life Sciences
-
Takahashi Noriko
Department of Molecular and Functional Genomics, Center for Integrated Research in Science, Shimane University
-
Tanaka Kyoko
Graduate School of Life and Environmental Sciences, Kyoto Prefectural University
-
Nakamura Shinya
Department of Molecular and Functional Genomics, Center for Integrated Research in Science, Shimane University
-
NAKAGAWA Tsuyoshi
Department of Materials Science and Engineering, Graduate School of Engineering, Nagoya Institute of Technology
関連論文
- Gateway Binary Vectors with the Bialaphos Resistance Gene, bar, as a Selection Marker for Plant Transformation
- Strontium Isotope Effects Observed in Liquid Chromatography with Crown Ether Resins
- MOLECULAR GENETICAL DISSECTION OF MECHANISMS OF SALT TOLERANCE IN PLANTS
- ARABIDOPSIS MUTANTS, rec, ces, AND des EXPRESSING THE PHOTOSYNTHESIS GENE RBCS IN DERANGED TISSUE-SPECIFICITY
- SELECTION AND ANALYSIS OF ARABIDOPSISMUTANTS UNDER-EXPRESSING RBCS
- In Planta Production of Immunogenic Poliovirus Peptide Using Tobacco Mosaic Virus-Based Vector System(PLANT BIOTECHNOLOGY)
- Production of Anti-Amyloid β Antibodies in Mice Fed Rice Expressing Amyloid β
- A Study on Zinc Isotope Fractionation in a Benzo Crown Resin/Acetone System
- Zinc Isotope Accumulation in Liquid Chromatography with Crown Ether Resin
- Novel Syntheses Method of Phenol Type Benzo-15-Crown-5 Ether Resin and its Application for Lithium Isotope Separation
- Zinc Isotope Separation by Phenol Formaldehyde Type 15-Crown-5 Resin in Organic Solvents
- Geranylgeranyl Diphosphate Synthase from Scoparia dulcis and Croton sublyratus. Plastid Localization and Conversion to a Farnesyl Diphosphate Synthase by Mutagenesis
- Two Novel Nuclear Genes, OsSIG5 and OsSIG6, Encoding Potential Plastid Sigma Factors of RNA Polymerase in Rice : Tissue-Specific and Light-Responsive Gene Expression
- NOVEL ces MUTANTS OF Arabidopsis thaliana IN WHICH ENDOGENOUS PHOTOSYNTHESIS GENE RBCS IS EXPRESSED IN CALLI INDUCED FROM ROOT TISSUES
- SELECTION OF ARABIDOPSIS MUTANTSEXPRESSING RBCS IN ROOTS
- Catalog of Micro-Tom tomato responses to common fungal, bacterial, and viral pathogens
- Development of series of gateway binary vectors, pGWBs, for realizing efficient construction of fusion genes for plant transformation
- -P85- RELATION BETWEEN TL-201 MYOCARDIAL SPECT FINDINGS AND PROGNOSIS IN DILATED CARDIOMYOPATHY : RI : FREE COMMUNICATIONS(I) : PROCEEDINGS OF THE 51th ANNUAL SCIENTIFIC MEETING OF THE JAPANESE CIRCULATION SOCIETY
- Arabidopsis Mutants by Activation Tagging in which Photosynthesis Genes are Expressed in Dedifferentiated Calli
- VISUALIZATION OF LIVING ORGANELLES IN Arabidopsis
- INDUCIBLE EXPRESSION SYSTEM MEDIATED BYTHE YEAST FLP/FRT SITE-SPECIFIC RECOMBINATION IN ARABIDOPSIS ANDMAIZE
- A Novel Selection Method Based on the Expression Level of Green Fluorescent Protein Measured with a Quantitative Fluorescence Imager
- A Synthetic Green Fluorescent Protein Gene for Plant Biotechnology
- Gateway vectors for plant transformation
- EVALUATION OF GENE PROMOTER STRENGTHS BY TRANSIENT EXPRESSION IN ARABIDOPSIS THALIANA CHLOROPLASTS
- Expression of a Gene for Cyclophilin Which Contains an Amino-Terminal Endoplasmic Reticulum-Targeting Signal
- A Nuclear Factor That Binds to a Dyad-Symmetric Sequence with a CGTCA Motif in the 5'-Upstream Region of the Sweet Potato β-Amylase Gene
- Transition of children with membranoproliferative glomerulonephritis to adolescence and adulthood
- Two cases of congenital nephrotic syndrome
- Long-Term Follow-up of Renal Transplantation in Children: A 20-Year Experience at One Kidney Center in Japan
- FOURIER ANALYSIS OF MULTI-GATED BLOOD-POOL DATA IN PATIENTS WITH CONGENITAL HEART DISEASE : PROCEEDINGS OF THE 47th ANNUAL SCIENTIFIC MEETING OF THE JAPANESE CIRCULATION SOCIETY : Radioisotope
- EVALUATION OF VENTRICULAR CONTRACTION ABNORMALITY USING MULTIPLE GATED BLOOD POOL SCINTIGRAPHY AND ITS FOURIER ANALYSIS : Radio isotope : FREE COMMUNICATIONS (Abstract) : 45 Annual Scientific Meeting, Japanese Circulation Society
- The Generation of Monoclonal Antibodies against Human Peroxisome Proliferator-activated Receptors(PPARs)
- Regulation of CAPRICE Transcription by MYB Proteins for Root Epidermis Differentiation in Arabidopsis
- The analysis of CPC gene in root hair formation in Arabidopsis thaliana
- Regulatory Systems of Root Patterning
- Enhanced Piezoelectric Property of Barium Titanate Single Crystals with Engineered Domain Configurations
- Change of Macroscopic and Microscopic Symmetry of Barium Titanate Single Crystal around Curie Temperature
- Measurement of Semiconductor Heterojunction Band Discontinuities Using Free Electron Laser
- A Cytokinin-Repressed Gene in Cucumber for a bHLH Protein Homologue is Regulated by Light
- Cloning and Sequence Analysis of Genes for Cyclophilin from Arabidopsis thaliana : GENE STRUCTURE AND EXPRESSION
- Development of Gateway Binary Vectors, R4L1pGWBs, for Promoter Analysis in Higher Plants
- Development of R4 Gateway Binary Vectors (R4pGWB) Enabling High-Throughput Promoter Swapping for Plant Research
- Enhancement of Electron Mobility in Quasi-One-Dimensional Structure
- Comparative Study of C-V and Transconductance of a Si δ-Doped GaAs FET Structure
- Picosecond Photoluminescence Study of Relaxation Phenomena of Hot Electrons in a Quasi-One-Dimensional Structure
- On the Electron Mobility in Quasi-One-Dimensional Structures Fabricated by Holographic Lithography and Wet Chemical Etching
- MOLECULAR GENETIC ANALYSIS OF A MALE-STERILE MUTANT, VW294,WHICH IS RESCUED BY HIGH-HUMIDITY
- MOLECULAR GENETIC ANALYSIS OF FLORAL ORGAN DEVELOPMENT AND GAMETOGENESIS USING T-DNA TAGGING MUTANTS
- SCREENING AND ANALYSIS OF ARABIDOPSIS MUTANTS GENERATED BY ACTIVATION TAGGING IN WHICH PHOTOSYNTHESIS GENES ARE EXPRESSED IN NONPHTOSYNTHETIC CALLI
- GENERATION OF TRANSGENIC Arabidopsis EXPRESSING ENGINEERED GFPs
- ENGINEERED GFP AS A VITAL REPORTER FOR PROTEIN LOCALIZATION IN PLANT CELLS
- Application of HfSiON to Deep Trench Capacitors of Sub-45nm Node Embedded DRAM
- Quantitative Analysis of Domain Walls in Magneto-Optic Thin Films By Magnetic Force Microscopy
- Optical Parametric Amplification using the Phase Matching Retracing Behavior in MgO:LiNbO_3 for Generation of Intense Widely Tunable Mid-infrared Pulses
- Synthesis of Diamond by DC Plasrna Chemical Vapor Deposition above the Surface of a Water-Ethylene Glycol Solution
- 酸化スズガスセンサ膜の微構造およびホ-ル効果の膜厚依存
- The Effect of the Particle Sizes and the Correlational Sizes of Dipoles Introduced by the Lattice Defects on the Crystal Structure of Barium Titanate Fine Particles
- 29a-YB-3 An intense picosecond mid-infrared light source and its application to sum frequency generation from surfaces
- Defect Structure of Heteroepitaxial SnO_2 Thin Films Grown on TiO_2 Substrates : Surfaces, Interfaces, and Films
- Local Epitaxial Growth of (103) One-Axis-Oriented SrBi_2Ta_2O_9 Thin Films Prepared at Low Deposition Temperature by Metalorganic Chemical Vapor Deposition and Their Electrical Properties
- Effect of Nonstoichiometry on Microstructure of Epitaxially Grown BaTiO_3 Thin Films
- Thermal Stability of Artificial SrTiO_3/SrO Superlattice Epitaxially Grown on SrTiO_3 Single Crystal
- Solid Solution of Epitaxially Grown(Sr, Mg)TiO_3 Thin Films
- Electrical Characterization of SrTiO_3 Thin Films Grown on Nb-Doped SrTiO_3 Single Crystals
- Artificial SrTiO_3/SrO Superlattices by Pulsed Laser Deposition
- Interface and Domain Structures of (116)-Oriented SrBi_2Ta_2O_9 Thin Film Epitaxially Grown on (110) SrTiO_3 Single Crystal
- Interface and Defect Structures of (001)-Oriented SrBi_2Ta_2O_9 Thin Film Epitaxially Grown on (001) SrTiO_3 Single Crystal
- Optical Confinement Effect for below 5 μm Thin Film Poly-Si Solar Cell on Glass Substrate
- A decrease in interleukin-1 receptor antagonist expression in the prefrontal cortex of schizophrenic patients
- 脳性麻痺による長期臥床を余儀なくされた続発性骨粗鬆症の現状
- Long-term follow-up of type II membranoproliferative glomerulonephritis in two children
- Loss of AtPDR8, a Plasma Membrane ABC Transporter of Arabidopsis thaliana, Causes Hypersensitive Cell Death Upon Pathogen Infection
- Zinc Transporter of Arabidopsis thaliana AtMTP1 is Localized to Vacuolar Membranes and Implicated in Zinc Homeostasis
- Quadrupolar Effect in Strongly Correlated Systems
- Use of Green Fluorescent Protein as a Molecular Tag of Protein Movement In vivo
- Light-Dependent Intracellular Positioning of Mitochondria in Arabidopsis thaliana Mesophyll Cells
- The Arabidopsis FLAKY POLLEN1 Gene Encodes a 3-Hydroxy-3-Methylglutaryl-Coenzyme A Synthase Required for Development of Tapetum-Specific Organelles and Fertility of Pollen Grains
- Identification of kaonashi Mutants Showing Abnormal Pollen Exine Structure in Arabidopsis thaliana
- -0324-CIRCADIAN CHARACTERISTICS OF VENTRICULAR ECTOPY (VE) IN CORONARY ARTERIAL DISEASE (CAD) AND MYOCARDIOPATHY (M)
- Location of a Possible miRNA Processing Site in SmD3/SmB Nuclear Bodies in Arabidopsis
- A virus-induced gene silencing approach for the suppression of nicotine content in Nicotiana benthamiana
- Use of an Attenuated Strain of Tobamovirus for Early Detection of Virus-Induced Gene Silencing
- Arabidopsis AUXIN RESPONSE FACTOR6 and 8 Regulate Jasmonic Acid Biosynthesis and Floral Organ Development via Repression of Class 1 KNOX Genes
- Development of R4 Gateway Binary Vectors (R4pGWB) Enabling High-Throughput Promoter Swapping for Plant Research
- Improved Gateway Binary Vectors : High-Performance Vectors for Creation of Fusion Constructs in Transgenic Analysis of Plants
- LIPO-MICRODIALYSIS : A NEW MICRODIALYSIS METHOD FOR STUDYING THE PHARMACOKINETICS OF LIPOPHILIC SUBSTANCES
- Differential Expression Control and Polarized Distribution of Plasma Membrane-Resident SYP1 SNAREs in Arabidopsis thaliana
- Development of Gateway Binary Vectors, R4L1pGWBs, for Promoter Analysis in Higher Plants
- Transformation of Arabidopsis with Plant-Derived DNA Sequences Necessary for Selecting Transformants and Driving an Objective Gene
- No association between the ERBB3 gene and schizophrenia in a Japanese population
- Gateway Binary Vectors with the Bialaphos Resistance Gene, bar, as a Selection Marker for Plant Transformation
- Production of Anti-Amyloid β Antibodies in Mice Fed Rice Expressing Amyloid β
- MAA3 (MAGATAMA3) Helicase Gene is Required for Female Gametophyte Development and Pollen Tube Guidance in Arabidopsis thaliana
- The tryptophan hydroxylase 1 (TPH1) gene and risk of schizophrenia : A moderate-scale case-control study and meta-analysis
- No associations exist between five functional polymorphisms in the catechol-O-methyltransferase gene and schizophrenia in a Japanese population
- Construction of a Tobamovirus Vector That Can Systemically Spread and Express Foreign Gene Products in Solanaceous Plants
- Context Analysis of Termination Codons in mRNA that are Recognized by Plant NMD
- The Mechanism Selecting the Guide Strand from Small RNA Duplexes is Different Among Argonaute Proteins
- Calcium isotope fractionation in liquid chromatography with crown ether resins