A virus-induced gene silencing approach for the suppression of nicotine content in Nicotiana benthamiana

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概要

• 論文の詳細を見る

• Virus vectors have been used to study plant gene function by transiently overexpressing specific gene products, or conversely, silencing specific endogenous gene functions. Previously, we reported the establishment of tobamovirus vectors and its applications. In this study, we observed that tobamovirus vector could drive ectopic expression of green fluorescent protein (GFP) in roots after infecting leaves, indicating successful invasion into underground tissues. Subsequently, we attempted to apply the tobamovirus vector system to suppress the expression of putrescine N-methyltransferase (PMT), which is the first committed key enzyme for the biosynthesis of nicotine and is active in the underground parts of plants. Two or three weeks after inoculation with the vector harboring a partial fragment of PMT cDNA, we observed a reduction in the PMT mRNA level in the root and in the nicotine content of the aerial parts of the plant Nicotina benthamiana. The possibilities and limitations of the virus vector for the analysis of metabolic pathways are discussed.

• 日本植物細胞分子生物学会の論文
• 2007-06-01

著者

• Takizawa Mari

  Department of Pediatrics, National Defense Medical College

• Watanabe Yuichiro

  Department of Life Science, Graduate School of Arts and Sciences, University of Tokyo

• Hashimoto Takashi

  Graduate School of Biological Sciences, Nara Institute of Science and Technology

• HORI Koichi

  Department of Life Science, Graduate School of Arts and Sciences, University of Tokyo

• TAKASE Hisabumi

  Graduate School of Biological Sciences, Nara Institute of Science and Techonology

• Hashimoto Takashi

  Graduate School Of Biological Sciences Nara Institute Of Science And Technology

• Hori K

  Department Of Life Sciences The University Of Tokyo

• Takizawa Mari

  Department Of Pediatrics St. Marianna University School Of Medicine Yokohama City Seibu Hospital

• Takizawa M

  Department Of Life Sciences The University Of Tokyo

• Takizawa M

  Aids Research Center National Institute Of Infectious Diseases

• Takase Hisabumi

  Graduate School Of Biological Sciences Nara Institute Of Science And Technology

• INAI Koji

  Graduate School of Biological Sciences, Nara Institute of Science and Technology

• Inai Koji

  Graduate School Of Biological Sciences Nara Institute Of Science And Technology

• Watanabe Yuichiro

  Department Of Life Sciences The University Of Tokyo

• Hori Koichi

  Department Of Life Science Graduate School Of Arts And Sciences University Of Tokyo

• Watanabe Yuichiro

  Department Of Life Science Graduate School Of Arts And Science The University Of Tokyo

• Takizawa Mari

  Department Of Pediatrics National Defense Medical College

• Hashimoto Takashi

  Graduate School Of Biol. Sci.

• Hashimoto Takashi

  Graduate School of Agricultural Science, Kobe University

• Takizawa Mari

  Department of Life Sciences, the University of Tokyo

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