Expression of the AtRAD51 Gene Promoter in Response to DNA Damage in Transgenic Tobacco
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概要
- 論文の詳細を見る
AtRAD51 gene encodes an Arabidopsis homologue of E. coli RecA protein that catalyzes homologous recombination and repair of chromosomal DNA. The AtRAD51 mRNA expression is regulated by DNA damage and cell cycle, but the mechanisms and signal transduction pathway involved in the AtRAD51 transcriptional regulation are largely unknown. In order to investigate regulatory mechanisms of DNA-damage induced gene expression in plants, we carried out functional analysis of the AtRAD51 gene promoter. A 0.7 kb fragment of the 5′-upstream region of AtRAD51 genomic DNA was fused to the firefly luciferase reporter gene and introduced into tobacco cells by microprojectile bombardment and Agrobacterium-mediated transformation. Induction experiment using bleomycin indicated that the AtRAD51 promoter is able to direct gene expression in tobacco cells in response to DNA damage.
- 日本植物細胞分子生物学会の論文
- 2004-06-01
著者
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Hiratsuka K
Graduate School Of Environment And Information Sciences Yokohama National University
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TAKASE Hisabumi
Graduate School of Biological Sciences, Nara Institute of Science and Techonology
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TAKASE Hisabumi
Grad. Sch. Biol. Sci.
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Watakabe Yuriko
Graduate School Of Biological Sciences Nara Institute Of Science And Technology
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MAEDA Tomohide
Division of Plant Biotechnology, Graduate School of Environment and Information Sciences, Yokohama N
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SEO Seunghee
Division of Plant Biotechnology, Graduate School of Environment and Information Sciences, Yokohama N
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HIRATSUKA Kazuyuki
Division of Plant Biotechnology, Graduate School of Environment and Information Sciences, Yokohama N
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MAEDA Tomohide
Grad. Sch. Biol. Sci.
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WATAKABE Yuriko
Grad. Sch. Biol. Sci.
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Seo Seunghee
Graduate School Of Environment And Information Sciences Yokohama National University
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Maeda Tomohide
Division Of Plant Biotechnology Graduate School Of Environment And Information Sciences Yokohama Nat
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Takase Hisabumi
Graduate School Of Biological Sciences Nara Institute Of Science And Technology
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Hiratsuka Kazuyuki
Division Of Plant Biotechnology Graduate School Of Environment And Information Sciences Yokohama Nat
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