Transient Assay System for the Analysis of PR-1a Gene Promoter in Tobacco BY-2 Cells
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概要
- 論文の詳細を見る
In order to develop a rapid and versatile assay system suitable for the analysis of regulated expression of tobacco pathogenesis-related protein 1a (PR-1a) gene, we investigated the use of the transient gene expression system in tobacco BY-2 cells by microprojectile bombardment. Using dual luciferase assay as a reporter gene expression detection system, we observed significant induction of PR-1a promoter activity by salicylic acid (SA) treatment. On the other hand, treatment with 4-hydroxybenzoic acid (4-HBA) resulted in no detectable increase in luciferase activity. Co-expression of a trans-acting factor, the NPR1/NIM1 protein of Arabidopsis, resulted in the induction of higher expression levels of the PR-1a promoter. These results suggest that the assay system is applicable for the analysis of factors involved in the regulated expression of SA-inducible defense-related genes.
- 社団法人 日本農芸化学会の論文
- 2004-04-23
著者
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Hiratsuka K
Graduate School Of Environment And Information Sciences Yokohama National University
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HIRATSUKA Kazuyuki
Graduate School of Biological Sciences, Nara Institute of Science and Techonology
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Hiratsuka Kazuyuki
Graduate School Of Biological Sciences Nara Institute Of Science And Technology
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Watakabe Yuriko
Graduate School Of Biological Sciences Nara Institute Of Science And Technology
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Ono Sachiko
Graduate School Of Environment And Information Sciences Yokohama National University
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TANAKA Tsuneyuki
Graduate School of Environment and Information Sciences, Yokohama National University
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Hiratsuka Kazuyuki
Division Of Plant Biotechnology Graduate School Of Environment And Information Sciences Yokohama Nat
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Tanaka Tsuneyuki
Graduate School Of Environment And Information Sciences Yokohama National University
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