Isolation of a Promoter that Directs Microsporogenesis-Associated Gene Expression in Lilium longiflorum
スポンサーリンク
概要
- 論文の詳細を見る
LIM genes, identified as cDNAs from pollen mother cells of Lillium longiflorum, are specifically induced at meiosis I of microsporogenesis. LIM8 encodes a protein which is highly homologous to amino acid transporters and exhibits high expression levels at late stages of microsporogenesis. To study the regulated expression of microsporogenesis-associated genes, we isolated a genomic clone containing the 5 upstream region of LIM8 gene by a PCR-based approach. Sequence analysis of the 0.6kb genomic DNA fragment revealed the presence of putative transcription factor binding sites. However, no sequence similarity was found in promoter regions of LIM8 gene and previously described meiosis-associated genes. Transient assay by microprojectile bombardment demonstrated that the putative promoter region of LIM8 gene is able to direct tissue-specific expression of the luciferase reporter gene in lily microsporocytes.
- 日本植物細胞分子生物学会の論文
- 2001-06-01
著者
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Hiratsuka K
Graduate School Of Environment And Information Sciences Yokohama National University
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TAKASE Hisabumi
Graduate School of Biological Sciences, Nara Institute of Science and Techonology
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HIRATSUKA Kazuyuki
Graduate School of Biological Sciences, Nara Institute of Science and Techonology
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TAKASE Hisabumi
Grad. Sch. Biol. Sci.
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Hiratsuka Kazuyuki
Graduate School Of Biological Sciences Nara Institute Of Science And Technology
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MINAMI Masayoshi
Graduate School of Biological Sciences, Nara Institute of Science and Technology
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Takase Hisabumi
Graduate School Of Biological Sciences Nara Institute Of Science And Technology
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Minami Masayoshi
Graduate School Of Biological Sciences Nara Institute Of Science And Technology
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Hiratsuka Kazuyuki
Division Of Plant Biotechnology Graduate School Of Environment And Information Sciences Yokohama Nat
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UEFUJI Hirotaka
Graduate School of Biological Sciences, Nara Institute of Science and Technology
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Uefuji Hirotaka
Graduate School Of Biological Sciences Nara Institute Of Science And Technology
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