Evaluation of the Use of the Tobacco PR-1a Promoter to Monitor Defense Gene Expression by the Luciferase Bioluminescence Reporter System
スポンサーリンク
概要
- 論文の詳細を見る
Because of their marked responsiveness to induction signals, genes encoding pathogenesis-related proteins are used as markers to monitor defense gene expression in plants. To develop a non-invasive bioluminescence reporter assay system, we tested acidic PR-1 gene promoters from tobacco and Arabidopsis. These two promoters share common regulatory elements and are believed to show similar responsiveness to various stimuli but the results of transient expression assays by microprojectile bombardment of various plant cells and npr1 mutant Arabidopsis suggest that the tobacco PR-1a promoter is superior to its Arabidopsis counterpart in terms of responsiveness to salicylic acid treatment. Transgenic Arabidopsis seedlings harboring the tobacco PR-1a promoter fused to firefly luciferase showed marked induction in response to treatment with chemicals that induce defense gene expression in plants. These results suggest that the tobacco PR-1a promoter is applicable in monitoring defense-gene expression in various plant species.
- 社団法人 日本農芸化学会の論文
著者
-
Hiratsuka Kazuyuki
Graduate School Of Biological Sciences Nara Institute Of Science And Technology
-
Ono Sachiko
Graduate School Of Environment And Information Sciences Yokohama National University
-
Ogura Rieko
Venture Business Laboratory, Yokohama National University
-
KUSAMA Masahiro
Graduate School of Environment and Information Sciences, Yokohama National University
関連論文
- Tissue-specific and DNA damage-responsive expression of the Arabidopsis RAD51 gene promoter in transgenic Arabidopsis and tobacco
- Characterization of a Novel GT-box Binding Protein from Arabidopsis
- Bioluminescence reporter assay system to monitor Arabidopsis MPK3 gene expression in response to infection by Botrytis cinerea
- Transient Assay System for the Analysis of PR-1a Gene Promoter in Tobacco BY-2 Cells
- Characterization of the EMCV-IRES Mediated Bicistronic Translation in Plant Cells
- Dual Luciferase Assay for Monitoring Transient Gene Expression in Higher Plants
- A high-throughput evaluation system for Arabidopsis mutants for defense signaling
- Isolation of a Promoter that Directs Microsporogenesis-Associated Gene Expression in Lilium longiflorum
- Characterization of a Meiosis - Associated Heat Shock Protein 70
- A Novel Glycine-Rich Protein is Associated with Starch Grain Accumulation during Anther Development
- Expression and subcellular localization of pre-rRNA processing factor homologues in higher plants
- Large-scale Sequencing of Meiosis-associated Genes from a cDNA Library of Lily Microsporocytes
- Fertility Investigation in F1 Hybrid and Backcross Progeny of Cattle (Bos taurus) and Yak (B. gruniens) in Mongolia. : II. Little variation in gene products studied in male sterile and fertile animals.
- Multi-color luciferases as reporters for monitoring transient gene expression in higher plants
- Bioluminescence spectra of click beetle luciferases in higher plant cells
- Non-destructive bioluminescence detection system for monitoring defense gene expression in tobacco BY-2 cells
- In vivo bioluminescence monitoring of defense gene expression in response to treatment with yeast cell wall extract
- Evaluation of the Use of the Tobacco PR-1a Promoter to Monitor Defense Gene Expression by the Luciferase Bioluminescence Reporter System
- Light Regulated Transcription in Higher Plants
- Non-destructive bioluminescence detection system for monitoring defense gene expression in tobacco BY-2 cells
- Bioluminescence spectra of click beetle luciferases in higher plant cells
- Evaluation of the Use of the Tobacco PR-1α Promoter to Monitor Defense Gene Expression by the Luciferase Bioluminescence Reporter System
- In vivo bioluminescence monitoring of defense gene expression in response to treatment with yeast cell wall extract
- Novel intron-containing luciferase genes for quantitative analysis of mRNA levels in transient gene expression assays
- Development of a promoter-luciferase-based high-throughput system to monitor jasmonate-mediated defense gene expression