Characterization of the EMCV-IRES Mediated Bicistronic Translation in Plant Cells
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概要
- 論文の詳細を見る
Internal ribosome entry sites (IRES) were first identified as sequences in viral genomes that allow cap-independent translation initiation. The IRES sequence from the encephalomyocarditis virus (EMCV) is able to direct internal translation initiation that allows co-expression of multiple genes from one mRNA. Although EMCV-IRES has also been demonstrated to be functional in transgenic plants, the characteristics of the EMCV-IRES in plant cells have not been fully defined. Using a transient expression system by microprojectile bombardment and quantitative reporter gene assay, we have analyzed EMCV-IRES activity in plant cells. We show that the spacing between the IRES sequence and the second cistron is crucial for IRES-dependent translation efficiency. However, the order of cistrons is not an important factor for the EMCV-IRES-dependent translation in plant cells. Studies in different plant species reveal species specificity for EMCV-IRES activity. Analysis of transgenic tobacco plants suggested that the EMCV-IRES-dependent translation is suppressed in roots.
- 日本植物細胞分子生物学会の論文
- 2004-06-01
著者
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GILMARTIN Philip
Centre for Plant Sciences, University of Leeds
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HIRATSUKA Kazuyuki
Graduate School of Biological Sciences, Nara Institute of Science and Techonology
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Hiratsuka Kazuyuki
Graduate School Of Environment And Information Sciences Yokohama National University
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Hiratsuka Kazuyuki
Graduate School Of Biological Sciences Nara Institute Of Science And Technology
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Gilmartin Philip
Centre For Plant Sciences Faculty Of Biological Sciences University Of Leeds
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MATSUO Naoko
Graduate School of Environment and Information Sciences, Yokohama National University
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Matsuo Naoko
Graduate School Of Environment And Information Sciences Yokohama National University
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Matsuo Naoko
Graduate School Of Biological Sciences Nara Institute Of Science And Technology
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