Identification of Novel Microsporogenesis-Associated Genes Encoding Proteins with a Nuclear Localization Signal
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概要
- 論文の詳細を見る
Microsporogenesis is a highly organized indispensable event for the sexual reproduction of higher plants. The process includes a series of organ development, cell differentiation and meiosis. We examined the functional genes involved in microsporogenesis by random sequencing using a cDNA library from lily zygotene stage microsporocytes and isolated two cDNAs encoding novel gene products. Deduced amino acid sequences of both gene products, designated M355 and M404 contained a cluster of basic amino acid residues that may constitute a nuclear loclization signal. RT-PCR analysis indicated that the temporal and spatial expression of M355 and M404 is associated with early stages of microsporogenesis or meiosis. Transient expression of GFP fusion proteins in onion epidermal cells revealed nuclear localization activity of both proteins. These results suggest that M355 and M404 are involved in a nuclear event during the progression of microsporogenesis in lily.
- 日本植物細胞分子生物学会の論文
- 2003-06-01
著者
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Hiratsuka K
Graduate School Of Environment And Information Sciences Yokohama National University
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Morohashi Kengo
Plant Biotechnology Center Ohio State University
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TAKASE Hisabumi
Graduate School of Biological Sciences, Nara Institute of Science and Techonology
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TAKASE Hisabumi
Grad. Sch. Biol. Sci.
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HIRATSUKA Kazuyuki
Division of Plant Biotechnology, Graduate School of Environment and Information Sciences, Yokohama N
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Takase Hisabumi
Graduate School Of Biological Sciences Nara Institute Of Science And Technology
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Hiratsuka Kazuyuki
Division Of Plant Biotechnology Graduate School Of Environment And Information Sciences Yokohama Nat
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BAYARMAA Gun-Aajav
Faculty of Biology, National University of Mongolia
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BAYARMAA Gun-Aajav
Graduate School of Biological Sciences, Nara Institute of Science and Technology
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MOROHASHI Kengo
Division of Plant Biotechnology, Graduate School of Environment and Information Sciences, Yokohama N
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Bayarmaa Gun
Faculty Of Biology National University Of Mongolia
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