Activation of SIPK in response to UV-C irradiation : utility of a glutathione-S transferase-tagged plant MAP kinase by transient expression with agroinfiltration
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概要
- 論文の詳細を見る
Salicylic acid (SA)-induced protein kinase (SIPK) in Nicotiana tabacum L. is activated in response to various stimuli. We assessed the response of SIPK in N. benthamiana. leaves to ultraviolet-C (UV-C) and SA stress. Infiltration of Agrobacterium tumefaciens EHA105 cells containing pBE2113-GST-SIPK into leaves gave transient production of glutathione-S transferase (GST)-fused SIPK. Irradiation of agroinfiltrated leaves with UV-C (254 nm) activated GST-SIPK and endogenous protein kinases with a molecular mass of about 45 kDa, close to that of SIPK simultaneously. These results suggest that SIPK is involved in stress signalling in Nicotiana plants under UV-C irradiation.
- 日本植物細胞分子生物学会の論文
- 2005-03-01
著者
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Yuasa Takashi
Kyushu Univ. Fukuoka Jpn
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Yuasa Takashi
Department Of Life Sciences Graduate School Of Arts And Sciences The University Of Tokyo
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Watanabe Yuichiro
Department Of Life Science Graduate School Of Arts And Science The University Of Tokyo
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Watanabe Yuichiro
Department Of Life Sciences Graduate School Of Arts And Sciences The University Of Tokyo
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SUGIKI Miyuki
Department of Plant Science, Graduate School of Science, The University of Tokyo
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Sugiki Miyuki
Department Of Plant Science Graduate School Of Science The University Of Tokyo
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Yuasa Takashi
Department of Bioresources Sciences, Faculty of Agriculture, Kyushu University
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