Isolation and characterization of a cDNA coding cowpea (Vigna unguiculata (L.) Walp.) calcineurin B-like protein-interacting protein kinase, VuCIPK1

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概要

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• We isolated a calcineurin B-like protein (CBL)-interacting protein kinase (EC 2.7.11.1) homolog (VuCIPK1) from cowpea (Vigna unguiculata (L.) Walp.) with significant similarity to AtCIPK3 (86% amino acid identity), which is involved in K+ transport. VuCIPK1 mRNA was detected in the whole plant by RT-PCR. Any significant change in VuCIPK1 mRNA levels was not detected when cowpea was subjected to various environmental stresses. Immunoblot assay with an anti-VuCIPK1 specific antibody and an anti-CBL antibody showed that immunologically CIPK- and CBL-related polypeptides were preferentially associated with membrane fractions of cowpea. The anti-VuCIPK1 antibody cross-reacted to two different CIPK-related polypeptides with relative molecular masses of 51 kDa in root and 55 kDa in mature leaf. Immunoprecipitation assay by anti-VuCIPK1 antibody using cowpea leaf extracts showed that endogenous VuCIPK1 was autophosphorylated on the threonine residues in response to salt stress. The recombinant VuCIPK1 phosphorylated casein in the presence of Mn2+. These observations suggest that membrane-associated VuCIPK1 is involved osmotic stress-activated kinase regulated by its phosphorylation status.

• 日本植物細胞分子生物学会の論文
• 2008-12-01

著者

• Shimazaki Ken‐ichiro

  Kyushu Univ. Fukuoka Jpn

• HTWE Nang

  Graduate school of Bioresource and Bioenvironmental Sciences, Kyushu University

• ZHENG Shao-Hui

  Coastal Bioenvironment Center, Saga University

• YUASA Takashi

  Department of Plant Resources, Faculty of Agriculture, Kyushu University

• IWAYA INOUE

  Department of Plant Resources, Faculty of Agriculture, Kyushu University

• SHIMAZAKI Ken-ichiro

  Department of Biology, Faculty of Science, Kyushu University

• Htwe Nang

  Graduate School Of Bioresource And Bioenvironmental Sciences Kyushu University

• Iwaya Inoue

  Department Of Plant Resources Faculty Of Agriculture Kyushu University

• Yamauchi Takashi

  Department Of Plant Resources Faculty Of Agriculture Kyushu University

• Zheng Shao-hui

  Coastal Bioenvironment Center Saga University

• Yamauchi Takashi

  Department Or Plant Resources Faculty Of Agriculture Kyushu University

• Iwaya Inoue

  Department Or Plant Resources Faculty Of Agriculture Kyushu University

• IMAMURA Masakazu

  Graduate School of Bioresource and Bioenvironmental Sciences, Kyushu University

• TAKAHASHI Tomoko

  Graduate School of Bioresource and Bioenvironmental Sciences, Kyushu University

• NAKAMURA Nagisa

  Graduate School of Bioresource and Bioenvironmental Sciences, Kyushu University

• Yuasa Takashi

  Department Of Life Sciences Graduate School Of Arts And Sciences The University Of Tokyo

• Shimazaki Ken-ichiro

  Department Of Biology Faculty Of Science Kyushu University

• Imamura Masakazu

  Graduate School Of Bioresource And Bioenvironmental Sciences Kyushu University

• Nakamura Nagisa

  Graduate School Of Bioresource And Bioenvironmental Sciences Kyushu University

• Iwaya Inoue

  Department Of Bioresources Sciences Faculty Of Agriculture Kyushu University

• Iwaya Inoue

  Department Of Bioresource Sciences Faculty Of Agriculture Kyushu University

• Zheng Shao-hui

  Coastal Biological Research Center Saga University

• Takahashi Tomoko

  Graduate School Of Bioresource And Bioenvironmental Sciences Kyushu University

• Yuasa Takashi

  Department of Bioresources Sciences, Faculty of Agriculture, Kyushu University

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